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Study Information
Study Attribute Value
CEBS Accession Number: 002-01013-0007-0000-4
Chemical Name: 1,1,1,2-Tetrachloroethane
CASRN: 630-20-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 472008
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 115 104 56 16 32
    86 103 116 45
    100 94 92 31
    98 99 105 36
Test Chemical 1,1,1,2-Tetrachloroethane 
12.5       
 93 81 100 36 34
    93 96 107 38
    100 94 80 27
  
25         
 86 79 65 25 26
    95 87 83 29
    100 79 68 23
  
50         
 107 94 70 22 29
    88 72 96 36
    109 84 93 29
  
100         
 96 77 128 44 37
    107 65 106 33
    112 100 110 33
  
200         
 81 36 90 37 31
    112 60 94 28
    108 51 91 28
  
300         
 40
    46 9 76 55
    105 26 80 25
  
500         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 83 57 210 85 62*
    90 53 178 66
    111 97 119 36

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 90 89 139 51 39
    97 113 107 37
    101 123 75 25
    63 76 84 45
Test Chemical 1,1,1,2-Tetrachloroethane 
50         
 79 62 92 39 37
    102 76 103 34
    100 85 118 39
  
75         
 92 79 84 30 36
    94 69 64 23
    60 42 96 54
  
100         
 93 50 88 32 36
    103 82 59 19
    50 55 84 57
  
150         
 92 41 72 26 36
    101 47 118 39
    87 49 115 44
  
200         
 52 16 94 60 50
    55 22 99 60
    58 16 54 31
Positive Control Methyl Methane Sulfonate 
5         
 97 66 647 222 259*
    111 69 980 295
    125r 75 786 210

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 104 117 109 35 38
    115 99 82 24
    63 77 118 63
    109 107 94 29
Test Chemical 1,1,1,2-Tetrachloroethane 
12.5       
 8r 8 106 424 75*
    44 45 131 100
    107 91 160 50
  
25         
 118 87 139 39 42
    109 99 136 42
    100 100 139 46
  
50         
 108 98 103 32 36
    105 98 137 43
    104 105 103 33
  
100         
 99 54 87 29 36
    116 84 129 37
    100 94 123 41
  
200         
 112 32 99 29 30
    115 66 102 30
    87 35 83 32
  
300         
   
  
400         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 120r 61 563 156 150*
    100 51 324 109
    84 38 482 192

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 115 79 141 41 41
    114 101 157 46
    122r 75 123 34
    114 120 123 36
Test Chemical 1,1,1,2-Tetrachloroethane 
12.5       
 116 47 153 44 41
    108 37 129 40
    109 99 126 39
  
25         
 119 62 144 40 37
    107 62 110 34
    101 67 108 36
  
50         
 105 77 142 45 42
    109# 64 129 39
    90 66 112 41
  
100         
 116 53 155 45 41
    114 92 106 31
    104 61 147 47
  
200         
 101 43 148 49 47
    112 52 152 45
  
300         
 111 24 128 38 31
    85 14 79 31
    114 21 85 25
Positive Control 3-Methylcholanthrene 
2.5       
 50 12 542 365 472*
    52 14 924 589
    48 12 665 463

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination