Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01013-0007-0000-4
Chemical Name:	1,1,1,2-Tetrachloroethane
CASRN:	630-20-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	472008
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	115	104	56	16	32
			86	103	116	45
			100	94	92	31
			98	99	105	36
Test Chemical	1,1,1,2-Tetrachloroethane	12.5	93	81	100	36	34
			93	96	107	38
			100	94	80	27
		25	86	79	65	25	26
			95	87	83	29
			100	79	68	23
		50	107	94	70	22	29
			88	72	96	36
			109	84	93	29
		100	96	77	128	44	37
			107	65	106	33
			112	100	110	33
		200	81	36	90	37	31
			112	60	94	28
			108	51	91	28
		300					40
			46	9	76	55
			105	26	80	25
		500


Positive Control	Methyl Methane Sulfonate	5	83	57	210	85	62*
			90	53	178	66
			111	97	119	36

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	90	89	139	51	39
			97	113	107	37
			101	123	75	25
			63	76	84	45
Test Chemical	1,1,1,2-Tetrachloroethane	50	79	62	92	39	37
			102	76	103	34
			100	85	118	39
		75	92	79	84	30	36
			94	69	64	23
			60	42	96	54
		100	93	50	88	32	36
			103	82	59	19
			50	55	84	57
		150	92	41	72	26	36
			101	47	118	39
			87	49	115	44
		200	52	16	94	60	50
			55	22	99	60
			58	16	54	31
Positive Control	Methyl Methane Sulfonate	5	97	66	647	222	259*
			111	69	980	295
			125r	75	786	210

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	104	117	109	35	38
			115	99	82	24
			63	77	118	63
			109	107	94	29
Test Chemical	1,1,1,2-Tetrachloroethane	12.5	8r	8	106	424	75*
			44	45	131	100
			107	91	160	50
		25	118	87	139	39	42
			109	99	136	42
			100	100	139	46
		50	108	98	103	32	36
			105	98	137	43
			104	105	103	33
		100	99	54	87	29	36
			116	84	129	37
			100	94	123	41
		200	112	32	99	29	30
			115	66	102	30
			87	35	83	32
		300

		400


Positive Control	3-Methylcholanthrene	2.5	120r	61	563	156	150*
			100	51	324	109
			84	38	482	192

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	115	79	141	41	41
			114	101	157	46
			122r	75	123	34
			114	120	123	36
Test Chemical	1,1,1,2-Tetrachloroethane	12.5	116	47	153	44	41
			108	37	129	40
			109	99	126	39
		25	119	62	144	40	37
			107	62	110	34
			101	67	108	36
		50	105	77	142	45	42
			109#	64	129	39
			90	66	112	41
		100	116	53	155	45	41
			114	92	106	31
			104	61	147	47
		200	101	43	148	49	47
			112	52	152	45	47
		300	111	24	128	38	31
			85	14	79	31
			114	21	85	25
Positive Control	3-Methylcholanthrene	2.5	50	12	542	365	472*
			52	14	924	589
			48	12	665	463

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination