Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01013-0008-0000-5
Chemical Name:	1,1,1,2-Tetrachloroethane
CASRN:	630-20-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	503956
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	118	102	111	31	35
			114	103	100	29
			109	96	122	37
			108	99	139	43
Test Chemical	1,1,1,2-Tetrachloroethane	12.5	123r	105	103	28
			113	89	103	30
		25	103	91	86	28	31
			95	77	95	33	31
		50	104	88	100	32	36
			98	78	115	39	36
		100	99	65	137	46	38
			106	72	96	30	38
		200	107	31	128	40	42
			94	33	121	43	42
Positive Control	Methyl Methane Sulfonate	20	26	10	197	251	319*
Positive Control			25	9	284	386	319*

Nonactivation Trial: 2 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	88	47	24	33
			79	107	88	37
			66	99	67	34
			79	106	87	37
Test Chemical	1,1,1,2-Tetrachloroethane	50	72	101	67	31	33
			68	102	71	35	33
		100	74	90	67	30	33
			76	88	79	35	33
		150	80	78	71	30	36
			78	85	100	43	36
		200	85	58	68	27	32
			83	61	94	38	32
		250	77#	26	102	44	51
			84	34	146	58	51
		300

Positive Control	Methyl Methane Sulfonate	20	5r	2	36	257
Positive Control			8r	3	68	302

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	104	80	31	41
			95	106	112	39
			104	95	96	31
			82	95	156	63
Test Chemical	1,1,1,2-Tetrachloroethane	100	84	83	79	31	33
			90	81	96	35	33
		150	100	61	96	32	37
			78	60	97	41	37
		200	77	41	95	41	41
			81	31	101	42	41
		250	93	23	146	52	54
			92	21	156	56	54
		300	71	5	386	180	196*
			55	4	348	212	196*
		350

Positive Control	Methyl Methane Sulfonate	20	13	4	96	253	236*
Positive Control			10	4	67	220	236*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	98	180	70	69
			79	103	167	71
			87	98	195	75
			80	101	143	60
Test Chemical	1,1,1,2-Tetrachloroethane	100	88	89	206	78	83
			82	67	212	87	83
		150	56	68	144	86	80
			70	60	155	74	80
		200	45	46	153	113	94
			64	52	145	76	94
		250	79	29	228	96	98
			72	34	218	100	98
		300	58	6	599	344	337*
			59	6	579	329	337*
		350

Positive Control	3-Methylcholanthrene	2.5	44	19	690	529	533*
Positive Control			38	18	609	537	533*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	104	139	64	63
			70	93	137	65
			66	101	126	64
			74#	101	133	60
Test Chemical	1,1,1,2-Tetrachloroethane	100	64	71	157	82	79
			61	65	138	75	79
		150	78	55	208	89	94
			58	47	173	99	94
		200	64	30	217	113	104*
			71	38	203	95	104*
		250	61	22	191	105	109*
			65	17	219	113	109*
		300	53	5	643	403	373*
			46	5	476	342	373*
		350

Positive Control	3-Methylcholanthrene	2.5	35	17	482	466	477*
Positive Control			34	19	495	488	477*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination