Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01028-0001-0000-4
Chemical Name:	1,2,3,4-Diepoxybutane DL
CASRN:	298-18-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	449785
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	71	103	68	32	29
			68	118	68	33
			77	95	41	18
			58	83	55	31
Test Chemical	1,2,3,4-Diepoxybutane DL	0.3125	55	81	80	49	50*
			64#	114	97	51	50*
		0.625	63	69	139	74	89*
			58#	61	181.5	104	89*
		1.25	34#	33	195	189	200*
			31	26	195	211	200*
		2.5	7r	3	70	318
			7r	2	76	390
		5

Positive Control	Ethyl Methane Sulfonate	250	70#	73	312	149	148*
Positive Control			80#	103	352.5	147	148*

Nonactivation Trial: 3 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	59	95	112	63	64
			72	92	152	70
			63	103	93	49
			71	110	154	72
Test Chemical	1,2,3,4-Diepoxybutane DL	0.4388	56	60	512	308	307*
			52	57	481	306	307*
		0.8775	28	16	730	885	1087*
			18	8	690	1290	1087*
		1.755	3r	1	195	1950
			4r	1	245	2130
		3.51

Positive Control	Ethyl Methane Sulfonate	250	43	61	408	319	282*
Positive Control			55	65	402	245	282*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination