Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01028-0002-0000-5
Chemical Name:	1,2,3,4-Diepoxybutane DL
CASRN:	298-18-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G05603
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	78	97	73	31	31
Vehicle Control			82	93	77	31	31
Test Chemical	1,2,3,4-Diepoxybutane DL	0.078	79#	81	227	95	94*
			91	76	250	92	94*
		0.156	66	55	345	175	158*
			85	68	364	142	158*
		0.313	48	43	413	286	303*
			50#	44	481.5	320	303*
		0.625	33	17	575	590	610*
			27	15	513	629	610*
		1.25	6r	1	225	1364
		2.5

Positive Control	Ethyl Methane Sulfonate	500	69#	54	815	397	382*
Positive Control			84	75	926	368	382*
Negative Control	Negative (Not Specified)	0	86	110	65	25

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	102	73	26	24
Vehicle Control			98#	100	63	22	24
Test Chemical	1,2,3,4-Diepoxybutane DL	0.078	102	117	160	52	53*
			90	110	143	53	53*
		0.156	99	106	188	63	64*
			87	92	166	64	64*
		0.313	51	50	283	187	155*
			61	61	225	123	155*
		0.625	44	30	396	303	331*
			41	24	435	358	331*
		1.25	18	5	237	435	548*
			11	2	225	662	548*
		2.5

Positive Control	Ethyl Methane Sulfonate	500	61	49	766	416	454*
Positive Control			52	39	773	491	454*
Negative Control	Negative (Not Specified)	0	78	83	66	28	25*
Negative Control			93	115	63	23	25*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	108	73	24	24
			98	89	66	22
			95	97	78	27
			99	106	62	21
Test Chemical	1,2,3,4-Diepoxybutane DL	0.156	82	65	152	62	58*
			96	74	158	55	58*
		0.313	75	68	182	81	79*
			90	57	211	78	79*
		0.625	70	56	290	139	143*
			77	60	341	147	143*
		1.25	89	33	530	199	234*
			67	29	540	269	234*
		1.5	10	1	266	858	810*
			17	4	396	762	810*
Positive Control	Ethyl Methane Sulfonate	500	57	27	1115	654	620*
Positive Control			66	33	1153	585	620*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	78	244	113	113
Vehicle Control			66	101	225	113	113
Test Chemical	1,2,3,4-Diepoxybutane DL	0.25	71	63	357	168	177
			70#	49	393	187	177
		0.5	61	49	457	249	236*
			58	66	393	224	236*
		1	28	22	283	341	331*
			35	27	337	321	331*
		2	17	10	265	530	589*
			14	5	275	647	589*
		4	2r	0	34	567

		8

Positive Control	3-Methylcholanthrene	5	48	17	576	403	411*
Positive Control			45	17	563	420	411*
Negative Control	Negative (Not Specified)	0	89	121	361	136

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	106	95	357	112	111
Vehicle Control			102	110	336	110	111
Test Chemical	1,2,3,4-Diepoxybutane DL	0.125	91	96	385	141	148
			79	98	366	154	148
		0.25	74	50	444	201	201*
			75#	66	448.5	200	201*
		0.5	100	79	536	178	214*
			70#	61	520.5	249	214*
		1	80	40	639	266	319*
			51	37	572	373	319*
		2	25	6	676	914	894*
			22	4	586	875	894*
		4

Positive Control	3-Methylcholanthrene	5	60	13	715	397	480*
Positive Control			40	9	682	564	480*
Negative Control	Negative (Not Specified)	0	100	94	311	104
Negative Control			122r	148	296	81

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination