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Study Information
Study Attribute Value
CEBS Accession Number: 002-01028-0003-0000-6
Chemical Name: 1,2,3,4-Diepoxybutane DL
CASRN: 298-18-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G68489
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Non-Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 78 99 80 34 32
    79 101 69 29
Test Chemical 1,2,3,4-Diepoxybutane DL 
 0.82      
 67 66 154 76 80*
    59 72 149 84
  
1.2       
 69 72 142 69 65*
    73 76 133 60
  
1.7       
 67 66 188 93 91*
    69 69 184 89
  
2.4       
 46 36 164 119 123*
    40 50 152 127
  
3.4       
 32 24 152 159 172*
    28 22 152 184
  
4.9       
 13 8 81 205 218*
    13 9 91 230
Positive Control 3-Methylcholanthrene 
6         
 55 49 242 146 156*
    56 45 276 165

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 96 99 268 93 91
    93 101 247 89
Test Chemical 1,2,3,4-Diepoxybutane DL 
 0.605     
 85 97 285 112 103
    110 101 313 95
  
 0.865     
 92 92 292 106 113
    83 84 300 120
  
1.24      
 85 86 314 123 116
    89 86 289 108
  
1.76      
 92 80 362 131 144
    59 54 277 157
  
2.52      
 67 57 266 132 127
    79 69 289 122
  
3.6       
 40 29 266 223 219*
    46 33 299 215
  
5.15      
 35 20 391 374 377*
    38 20 427 380
  
7.35      
 19 8 313 554 536*
    21 8 318 517
  
10.5       
 6r 2 187 1069
    7r 2 211 1029
  
15         
 0r 0 14 2800
Positive Control 3-Methylcholanthrene 
5         
 30 10 412 463 458*
    33 8 442 453

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 108 100 207 64 74
    99 92 219 74
    95 96 236 83
    117 113 259 74
Test Chemical 1,2,3,4-Diepoxybutane DL 
6.05      
 125r 121 300 80
    113 120 263 78
  
8.65      
 93 97 271 97 101
    110 79 345 104
  
12.4       
 91 93 380 139 129*
    119 95 423 119
  
17.6       
 97 87 438 151 149*
    89 87 391 146
  
25.2       
 88 81 462 175 224*
    72 58 587 272
  
36         
 63 39 642 338 346*
    62 46 660 355
  
51.5       
 37 17 655 593 557*
    37 20 579 522
  
73.5       
 16# 6 592.5 1274 1211*
    16 6 545 1147
  
105         
 5r 1 124 775
    5r 1 112.5 750
Positive Control 3-Methylcholanthrene 
5         
 64 40 731 384 350*
    61 38 574 315

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination