Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01028-0003-0000-6
Chemical Name:	1,2,3,4-Diepoxybutane DL
CASRN:	298-18-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G68489
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	78	99	80	34	32
Vehicle Control			79	101	69	29	32
Test Chemical	1,2,3,4-Diepoxybutane DL	0.82	67	66	154	76	80*
			59	72	149	84	80*
		1.2	69	72	142	69	65*
			73	76	133	60	65*
		1.7	67	66	188	93	91*
			69	69	184	89	91*
		2.4	46	36	164	119	123*
			40	50	152	127	123*
		3.4	32	24	152	159	172*
			28	22	152	184	172*
		4.9	13	8	81	205	218*
			13	9	91	230	218*
Positive Control	3-Methylcholanthrene	6	55	49	242	146	156*
Positive Control			56	45	276	165	156*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96	99	268	93	91
Vehicle Control			93	101	247	89	91
Test Chemical	1,2,3,4-Diepoxybutane DL	0.605	85	97	285	112	103
			110	101	313	95	103
		0.865	92	92	292	106	113
			83	84	300	120	113
		1.24	85	86	314	123	116
			89	86	289	108	116
		1.76	92	80	362	131	144
			59	54	277	157	144
		2.52	67	57	266	132	127
			79	69	289	122	127
		3.6	40	29	266	223	219*
			46	33	299	215	219*
		5.15	35	20	391	374	377*
			38	20	427	380	377*
		7.35	19	8	313	554	536*
			21	8	318	517	536*
		10.5	6r	2	187	1069
			7r	2	211	1029
		15	0r	0	14	2800
Positive Control	3-Methylcholanthrene	5	30	10	412	463	458*
Positive Control			33	8	442	453	458*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	108	100	207	64	74
			99	92	219	74
			95	96	236	83
			117	113	259	74
Test Chemical	1,2,3,4-Diepoxybutane DL	6.05	125r	121	300	80
			113	120	263	78
		8.65	93	97	271	97	101
			110	79	345	104	101
		12.4	91	93	380	139	129*
			119	95	423	119	129*
		17.6	97	87	438	151	149*
			89	87	391	146	149*
		25.2	88	81	462	175	224*
			72	58	587	272	224*
		36	63	39	642	338	346*
			62	46	660	355	346*
		51.5	37	17	655	593	557*
			37	20	579	522	557*
		73.5	16#	6	592.5	1274	1211*
			16	6	545	1147	1211*
		105	5r	1	124	775
			5r	1	112.5	750
Positive Control	3-Methylcholanthrene	5	64	40	731	384	350*
Positive Control			61	38	574	315	350*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination