Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01038-0009-0000-3
Chemical Name:	1,2,3-Trichloropropane
CASRN:	96-18-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	544452
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	103	112	201	65	60
			83	79	142	57
			99	103	166	56
			87	106	162	62
Test Chemical	1,2,3-Trichloropropane	0.0078	100	119	168	56	64
			78	92	155	66
			81	90	166	68
		0.0156	102#	117	130	42	51
			97	111	142	49
			93	102	171	62
		0.0313	86	108	137	53	71
			92	111	138	50
			69#	87	229	111
		0.0625	79	76	135	57	51
			84	98	114	46
			80	89	123	51
		0.125	99	70	181	61	61
			100#	74	187	62
			84	75	149	59
		0.25	90#	29	196	73	67
			86	49	159	62	67
		0.5


Positive Control	Ethyl Methane Sulfonate	250	52#	35	949	603	671*
			55	45	1182	714
			54	37	1133	697

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	117	133	127	36	46
			76	92	115	50
			83	92	113	45
			97	83	148	51
Test Chemical	1,2,3-Trichloropropane	0.0156	72	90	81	37	34
			70	85	61	29
			95	89	105	37
		0.0313	68	59	96	47	38
			65	68	70	36
			96	75	92	32
		0.0625	106	77	129	41	43
			85	70	129	50
			72	62	82	38
		0.125	76	43	118	52	43
			85	75	92	36
			87	66	110	42
		0.25	90	31	111	41	41
			74	25	109	49
			99	37	97	33
		0.5	58	9	140	80	103*
			45	8	168	125	103*
Positive Control	Ethyl Methane Sulfonate	250	83	45	796	318	379*
			85	50	1056	414
			81	47	987	405

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	83	104	78	31	36
			116	107	125	36
			108	108	111	34
			76	81	95	41
Test Chemical	1,2,3-Trichloropropane	1.56	69	106	80	38	32
			94	111	91	32
			76	98	59	26
		3.13	66	99	72	37	37
			83	129	91	36
			79	124	89	38
		6.25	77	130	81	35	38
			96	115	122	42
			99	124	105	35
		12.5	91#	106	257	95	98*
			82	75	228	93
			87	107	275	105
		25	73	64	546	250	204*
			92	79	505	182
			89	90	482	181
		50	39	12	741	628	612*
			44	18	721	550
			37	15	734	658
		100


Positive Control	3-Methylcholanthrene	2.5	89	76	621	233	223*
			79	63	587	248
			91	77	512	189

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	67	114	66	33	30
			77#	97	71	31
			72	90	56	26
Test Chemical	1,2,3-Trichloropropane	0.01	60#	87	85	47	48*
			55	78	73	44
			52	65	79	51
		0.02	67#	70	173	86	97*
			55	74	161	98
			52	70	169	109
		0.03	55	57	225	136	124*
			78#	89	166	71
			59	59	294	166
		0.04	71#	47	353	165	258*
			56	37	546	328
			55	41	464	280
		0.05	49	24	499	338	346*
			57	31	524	307
			45	25	532	393
		0.06	59	26	574	325	440*
			36	10	578	543
			32	8	436	452
Positive Control	3-Methylcholanthrene	2.5	83#	92	321	129	136*
Positive Control			77#	84	329	143	136*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination