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Study Information
Study Attribute Value
CEBS Accession Number: 002-01038-0009-0000-3
Chemical Name: 1,2,3-Trichloropropane
CASRN: 96-18-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 544452
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol
 0         
103 112 201 65 60
    83 79 142 57
    99 103 166 56
    87 106 162 62
Test Chemical 1,2,3-Trichloropropane
 0.0078    
100 119 168 56 64
    78 92 155 66
    81 90 166 68
 
 0.0156    
102# 117 130 42 51
    97 111 142 49
    93 102 171 62
 
 0.0313    
86 108 137 53 71
    92 111 138 50
    69# 87 229 111
 
 0.0625    
79 76 135 57 51
    84 98 114 46
    80 89 123 51
 
 0.125     
99 70 181 61 61
    100# 74 187 62
    84 75 149 59
 
 0.25      
90# 29 196 73 67
    86 49 159 62
 
 0.5       
   
   
Positive Control Ethyl Methane Sulfonate
250         
52# 35 949 603 671*
    55 45 1182 714
    54 37 1133 697

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
uL/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol
 0         
117 133 127 36 46
    76 92 115 50
    83 92 113 45
    97 83 148 51
Test Chemical 1,2,3-Trichloropropane
 0.0156    
72 90 81 37 34
    70 85 61 29
    95 89 105 37
 
 0.0313    
68 59 96 47 38
    65 68 70 36
    96 75 92 32
 
 0.0625    
106 77 129 41 43
    85 70 129 50
    72 62 82 38
 
 0.125     
76 43 118 52 43
    85 75 92 36
    87 66 110 42
 
 0.25      
90 31 111 41 41
    74 25 109 49
    99 37 97 33
 
 0.5       
58 9 140 80 103*
    45 8 168 125
Positive Control Ethyl Methane Sulfonate
250         
83 45 796 318 379*
    85 50 1056 414
    81 47 987 405

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol
 0         
83 104 78 31 36
    116 107 125 36
    108 108 111 34
    76 81 95 41
Test Chemical 1,2,3-Trichloropropane
1.56      
69 106 80 38 32
    94 111 91 32
    76 98 59 26
 
3.13      
66 99 72 37 37
    83 129 91 36
    79 124 89 38
 
6.25      
77 130 81 35 38
    96 115 122 42
    99 124 105 35
 
12.5       
91# 106 257 95 98*
    82 75 228 93
    87 107 275 105
 
25         
73 64 546 250 204*
    92 79 505 182
    89 90 482 181
 
50         
39 12 741 628 612*
    44 18 721 550
    37 15 734 658
 
100         
   
   
Positive Control 3-Methylcholanthrene
2.5       
89 76 621 233 223*
    79 63 587 248
    91 77 512 189

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
uL/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol
 0         
67 114 66 33 30
    77# 97 71 31
    72 90 56 26
Test Chemical 1,2,3-Trichloropropane
 0.01      
60# 87 85 47 48*
    55 78 73 44
    52 65 79 51
 
 0.02      
67# 70 173 86 97*
    55 74 161 98
    52 70 169 109
 
 0.03      
55 57 225 136 124*
    78# 89 166 71
    59 59 294 166
 
 0.04      
71# 47 353 165 258*
    56 37 546 328
    55 41 464 280
 
 0.05      
49 24 499 338 346*
    57 31 524 307
    45 25 532 393
 
 0.06      
59 26 574 325 440*
    36 10 578 543
    32 8 436 452
Positive Control 3-Methylcholanthrene
2.5       
83# 92 321 129 136*
    77# 84 329 143

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination