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Study Information
Study Attribute Value
CEBS Accession Number: 002-01044-0001-0000-2
Chemical Name: 1,2-Dibromo-4-(1,2-dibromoethyl)cyclohexane
CASRN: 3322-93-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 027958_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 11/06/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1043 476 0.456 9.52 26.5 0.000
Test Chemical 1,2-Dibromo-4-(1,2-dibromoethyl)cyclohexane 
20         
 50 1046 515 0.492 10.30 26.5 7.883 
30         
 50 1051 574 0.546 11.48 26.5 19.670 
40         
 50 1048 602 0.574 12.04 30.5 25.867
Positive Control Mitomycin-C 
 0.002     
 50 1048 831 0.793 16.62 26.5 73.747
Mitomycin-C 
 0.01      
 10 208 348 1.673 34.80 26.5 266.601
Trend: 4.121        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 12/06/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 503 0.481 10.06 26.5 0.000
Test Chemical 1,2-Dibromo-4-(1,2-dibromoethyl)cyclohexane 
10         
 50 1045 552 0.528 11.04 26.5 9.847 
20         
 50 1043 585 0.561 11.70 26.5 16.637 
30         
 50 1046 529 0.506 10.58 30.5 5.169
Positive Control Mitomycin-C 
 0.002     
 50 1048 788 0.752 15.76 26.5 56.361
Mitomycin-C 
 0.01      
 10 211 392 1.858 39.20 26.5 286.338
Trend: 1.257        
Probability: 0.104        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 11/06/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1042 566 0.543 11.32 26.0 0.000
Test Chemical 1,2-Dibromo-4-(1,2-dibromoethyl)cyclohexane 
50         
 50 1049 669 0.638 13.38 26.0 17.409 
75         
 50 1042 804 0.772 16.08 26.0 42.049 
100         
 50 1052 783 0.744 15.66 30.0 37.024
Positive Control Cyclophosphamide 
 0.5       
 50 1045 715 0.684 14.30 26.0 25.962
Cyclophosphamide 
2.5       
 10 207 316 1.527 31.60 26.0 181.040
Trend: 6.649        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 12/20/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 483 0.460 9.66 26.0 0.000
Test Chemical 1,2-Dibromo-4-(1,2-dibromoethyl)cyclohexane 
50         
 50 1043 673 0.645 13.46 26.0 40.139 
75         
 50 1044 636 0.609 12.72 26.0 32.308 
100         
 50 1043 738 0.708 14.76 26.0 53.674
Positive Control Cyclophosphamide 
 0.5       
 50 1046 792 0.757 15.84 26.0 64.445
Cyclophosphamide 
2.5       
 10 210 328 1.562 32.80 26.0 239.221
Trend: 6.450        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 01/08/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 360 0.344 7.20 26.5 0.000
Test Chemical 1,2-Dibromo-4-(1,2-dibromoethyl)cyclohexane 
30         
 50 1041 479 0.460 9.58 26.5 33.822 
50         
 50 1047 529 0.505 10.58 26.5 46.944 
100         
 50 1046 536 0.512 10.72 26.5 49.031
Positive Control Cyclophosphamide 
 0.5       
 50 1043 577 0.553 11.54 26.0 60.892
Cyclophosphamide 
2.5       
 10 209 241 1.153 24.10 26.0 235.363
Trend: 5.824        
Probability: 0.000