Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01044-0004-0000-5
Chemical Name:	1,2-Dibromo-4-(1,2-dibromoethyl)cyclohexane
CASRN:	3322-93-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	825772
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	81	107	104	43	38
			87	96	90	35
			89	101	101	38
			89	96	93	35
Test Chemical	1,2-Dibromo-4-(1,2-dibromoethyl)cyclohexane	6.25	94	95	97	34	37
			84	92	99	39	37
		12.5	72	55	64	30	32
			81	92	84	34	32
		25	60	65	69	38	43
			76	75	110	48	43
		50	23	2	590	861	837*
			30	3	724	813	837*
		100

Positive Control	Methyl Methane Sulfonate	20	23	11	188	271	240*
			30	10	190	210	240*
	Methyl Methane Sulfonate	250	63	79	349	185	203*
			61	62	405	222	203*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	98	78	33	28
			72	99	45	21
			68	91	56	27
			66	112	61	31
Test Chemical	1,2-Dibromo-4-(1,2-dibromoethyl)cyclohexane	10	59	106	55	31	41
			54	96	82	51	41
		20	88	115	57	22	26
			58	73	54	31	26
		30	65	74	48	25	37
			67	79	101	50	37
		40	67	36	119	59	68*
			64	27	149	77	68*
		50	57	8	242	142	141*
			52	9	220	140	141*
		60

Positive Control	Methyl Methane Sulfonate	20	16	10	115	245	251*
Positive Control			15	10	113	257	251*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination