Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01045-0015-0000-8
Chemical Name:	1,2-Dibromoethane
CASRN:	106-93-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G50253
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75	93	77	34	37
Vehicle Control			73	107	88	40	37
Test Chemical	1,2-Dibromoethane	0.04	72	74	166	76	81*
			80	73	202	85	81*
		0.05	76	70	209	92	104*
			65	62	227	117	104*
		0.07	66	59	226	114	116*
			69	59	244	118	116*
		0.08	66	46	452	230	217*
			58	45	353	205	217*
		0.1	44	29	308	236	202*
			57	39	285	168	202*
		0.13	41	19	436	356	321*
			43	20	368	285	321*
Positive Control	Ethyl Methane Sulfonate	500	43	39	690	537	508*
Positive Control			50	40	720	478	508*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	99	73	32	24
Vehicle Control			86	101	44	17	24
Test Chemical	1,2-Dibromoethane	0.05	91	77	204	75	98*
			59	55	217	122	98*
		0.07	72	62	185	85	103*
			68	59	245	120	103*
		0.08	68	45	287	141	134*
			72	51	273	127	134*
		0.1	54	33	234	146	154*
			54	30	263	162	154*
		0.13	44#	22	313.5	236
		0.16	24	7	263	368	344*
			29	9	275	320	344*
		0.2	10#	1	151.5	505	438*
			12	2	128	371	438*
Positive Control	Ethyl Methane Sulfonate	500	55	44	509	307	307*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	98	230	81	73
			94	108	224	80
			98	99	181	61
			76	96	157	69
Test Chemical	1,2-Dibromoethane	0.005	85	66	231	90	95
			93	74	278	100	95
		0.008	109	87	353	108	119*
			83	71	325	130	119*
		0.013	101	57	570	188	207*
			75	29	513	227	207*
		0.022	58	11	775	447	471*
			45	12	671	495	471*
		0.037

Positive Control	3-Methylcholanthrene	5	91	75	693	254	264*
			87	27	657	252
			67	53	577	286

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	115	325	124	119
			84	97	302	119
			84	100	282	113
			80	88	291	122
Test Chemical	1,2-Dibromoethane	0.008	106#	87	784.5	246	215*
			98	80	544	185	215*
		0.01	64	65	588	307	284*
			69	69	543	262	284*
		0.012	52	36	631	407	378*
			55	41	572	350	378*
		0.015	37	15	566	505	458*
			38	18	465	410	458*
		0.019	16	3	388	800	748*
			15	2	303	697	748*
		0.024

Positive Control	3-Methylcholanthrene	5	48	41	667	465	430*
			67	51	714	355
			48	42	681	470

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination