Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01045-0016-0000-9
Chemical Name:	1,2-Dibromoethane
CASRN:	106-93-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G62050
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	62	50	24	23
			65	78	42	22
			73	149	48	22
			114	112	84	25
Test Chemical	1,2-Dibromoethane	0.0313	83	70	160	65	62*
			101	90	179	59	62*
		0.0625	92	66	255	92	118*
			72	56	312	144	118*
		0.125	62	22	547	293	312*
			58	12	575	331	312*
		0.188	16	2	296	610	579*
			29	5	484	547	579*
		0.25
			6r	0	113	646
		0.375

Positive Control	Ethyl Methane Sulfonate	500	51	36	725	472	567*
			39	36	714	613
			37	31	691	617

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	67	107	43	21	21
			57	77	50	29
			57	117	43	25
			57	99	16	9
Test Chemical	1,2-Dibromoethane	0.0078	72	111	25	12	11
			73	156	23	11	11
		0.0156	66	81	34	17	19
			61	97	38	21	19
		0.0313	57	72	37	22	24
			69	86	53	26	24
		0.0625	65	74	70	36	34
			58	74	55	32	34
		0.125	40	27	92	76	67*
			36	25	63	59	67*
		0.25	12	2	62	175	172*
			9r	1	44	169	172*
		0.5

Positive Control	Ethyl Methane Sulfonate	500	33	35	550	553	472*
			37	31	555	498
			41	47	448	366

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	114	32	15	17
			80	98	37	15
			55	87	37	22
			64	100	27	14
Test Chemical	1,2-Dibromoethane	0.025	68	89	45	22	28
			66	88	68	34	28
		0.05	61	63	106	58	54*
			66	85	98	49	54*
		0.075	48	37	194	136	140*
			42	35	178	143	140*
		0.1	48	41	190	132	118*
			42	36	131	104	118*
		0.15	31	18	151	161	144*
			23	13	88	126	144*
		0.2	12	2	63	177	242*
			8r	2	75	306	242*
Positive Control	Ethyl Methane Sulfonate	500	35	29	797	770	733*
			28	32	658	798
			39	42	740	632

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	99	124	204	69	82
			102	92	276	90
			100	84	264	88
Test Chemical	1,2-Dibromoethane	0.002	102	126	247	81	85
			105	97	281	89	85
		0.0039	102	72	356	116	114
			99	79	332	112	114
		0.0078	103	44	540	175	170*
			84	41	411	164	170*
		0.0117	83	27	456	183	196*
			77	18	484	210	196*
		0.0156	57	14	476	279	292*
			38#	6	346.5	305	292*
		0.0235
		0.0313

Positive Control	3-Methylcholanthrene	5	40	8	389	326	382*
			39	9	415	356
			17	4	239	464

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	91	50	19	31
			89	75	61	23
			104	104	130	42
			102	129	121	39
Test Chemical	1,2-Dibromoethane	0.001	92	93	43	16	22
			94	66	82	29	22
		0.002	102	81	172	56	55*
			80	87	128	54	55*
		0.0038	77	88	167	72	62*
			97	97	151	52	62*
		0.0075	108	76	216	67	42
			92	71	47	17	42
		0.01	84	65	44	17	21
			98	74	73	25	21
		0.02	97	55	391	134	132*
			101	47	391	130	132*
Positive Control	3-Methylcholanthrene	5	39	20	374	317	278*
			64	25	616	323
			76	27	444	194

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	87	102	41	43
			82	93	90	37
			67	106	110	55
			76	114	91	40
Test Chemical	1,2-Dibromoethane	0.0025	140r	120	131	31
			91	76	195	72
		0.005	73	43	218	100	83*
			95	72	189	66	83*
		0.0075	76	104	95	41	39
			92	103	103	37	39
		0.01	61	25	233	128	106*
			70	46	175	84	106*
		0.015	48	11	243	169	217*
			41	5	325	266
			40	7	256	214
		0.02

			46	7	217	156
		0.03

Positive Control	3-Methylcholanthrene	5	33	11	373	373	419*
			25	9	383	514
			31	13	341	369

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination