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Study Information
Study Attribute Value
CEBS Accession Number: 002-01045-0016-0000-9
Chemical Name: 1,2-Dibromoethane
CASRN: 106-93-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G62050
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 69 62 50 24 23
    65 78 42 22
    73 149 48 22
    114 112 84 25
Test Chemical 1,2-Dibromoethane 
 0.0313    
 83 70 160 65 62*
    101 90 179 59
  
 0.0625    
 92 66 255 92 118*
    72 56 312 144
  
 0.125     
 62 22 547 293 312*
    58 12 575 331
  
 0.188     
 16 2 296 610 579*
    29 5 484 547
  
 0.25      
    6r 0 113 646
  
 0.375     
   
Positive Control Ethyl Methane Sulfonate 
500         
 51 36 725 472 567*
    39 36 714 613
    37 31 691 617

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 67 107 43 21 21
    57 77 50 29
    57 117 43 25
    57 99 16 9
Test Chemical 1,2-Dibromoethane 
 0.0078    
 72 111 25 12 11
    73 156 23 11
  
 0.0156    
 66 81 34 17 19
    61 97 38 21
  
 0.0313    
 57 72 37 22 24
    69 86 53 26
  
 0.0625    
 65 74 70 36 34
    58 74 55 32
  
 0.125     
 40 27 92 76 67*
    36 25 63 59
  
 0.25      
 12 2 62 175 172*
    9r 1 44 169
  
 0.5       
   
Positive Control Ethyl Methane Sulfonate 
500         
 33 35 550 553 472*
    37 31 555 498
    41 47 448 366

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 71 114 32 15 17
    80 98 37 15
    55 87 37 22
    64 100 27 14
Test Chemical 1,2-Dibromoethane 
 0.025     
 68 89 45 22 28
    66 88 68 34
  
 0.05      
 61 63 106 58 54*
    66 85 98 49
  
 0.075     
 48 37 194 136 140*
    42 35 178 143
  
 0.1       
 48 41 190 132 118*
    42 36 131 104
  
 0.15      
 31 18 151 161 144*
    23 13 88 126
  
 0.2       
 12 2 63 177 242*
    8r 2 75 306
Positive Control Ethyl Methane Sulfonate 
500         
 35 29 797 770 733*
    28 32 658 798
    39 42 740 632

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 99 124 204 69 82
    102 92 276 90
    100 84 264 88
Test Chemical 1,2-Dibromoethane 
 0.002     
 102 126 247 81 85
    105 97 281 89
  
 0.0039    
 102 72 356 116 114
    99 79 332 112
  
 0.0078    
 103 44 540 175 170*
    84 41 411 164
  
 0.0117    
 83 27 456 183 196*
    77 18 484 210
  
 0.0156    
 57 14 476 279 292*
    38# 6 346.5 305
  
 0.0235    
  
 0.0313    
   
Positive Control 3-Methylcholanthrene 
5         
 40 8 389 326 382*
    39 9 415 356
    17 4 239 464

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 90 91 50 19 31
    89 75 61 23
    104 104 130 42
    102 129 121 39
Test Chemical 1,2-Dibromoethane 
 0.001     
 92 93 43 16 22
    94 66 82 29
  
 0.002     
 102 81 172 56 55*
    80 87 128 54
  
 0.0038    
 77 88 167 72 62*
    97 97 151 52
  
 0.0075    
 108 76 216 67 42
    92 71 47 17
  
 0.01      
 84 65 44 17 21
    98 74 73 25
  
 0.02      
 97 55 391 134 132*
    101 47 391 130
Positive Control 3-Methylcholanthrene 
5         
 39 20 374 317 278*
    64 25 616 323
    76 27 444 194

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 87 102 41 43
    82 93 90 37
    67 106 110 55
    76 114 91 40
Test Chemical 1,2-Dibromoethane 
 0.0025    
 140r 120 131 31
    91 76 195 72
  
 0.005     
 73 43 218 100 83*
    95 72 189 66
  
 0.0075    
 76 104 95 41 39
    92 103 103 37
  
 0.01      
 61 25 233 128 106*
    70 46 175 84
  
 0.015     
 48 11 243 169 217*
    41 5 325 266
    40 7 256 214
  
 0.02      
   
    46 7 217 156
  
 0.03      
   
Positive Control 3-Methylcholanthrene 
5         
 33 11 373 373 419*
    25 9 383 514
    31 13 341 369

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination