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Study Information
Study Attribute Value
CEBS Accession Number: 002-01048-0010-0000-6
Chemical Name: 1,2-Dichloropropane (propylene dichloride)
CASRN: 78-87-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 552580
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 115 109 74 21 25
    107 95 90 28
    86 96 55 21
    85 100 74 29
Test Chemical 1,2-Dichloropropane (propylene dichloride) 
62.5       
 84 83 64 26 23
    78 88 53 23
    86 99 57 22
  
125         
 96 73 72 25 24
    81 68 58 24
    85 73 62 24
  
250         
 85 63 67 26 26
    79# 79 72 31
    114 76 72 21
  
500         
 94 69 44 16 21
    88 58 75 29
    76 56 45 20
  
750         
 74 36 70 31 31
    63 46 58 31
    71 35 65 31
  
1000         
 50 8 52 35 34
    78 24 65 28
    81 29 96 40
Positive Control Methyl Methane Sulfonate 
5         
 49 39 268 182 170*
    62 60 296 160
    48 48 243 168

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 113 97 88 26 26
    106 89 87 27
    108 96 79 24
    108 118 83 26
Test Chemical 1,2-Dichloropropane (propylene dichloride) 
62.5       
 114 98 91 27 26
    107 92 91 28
    117 119 79 23
  
125         
 113 81 84 25 26
    110 85 98 30
    109 90 75 23
  
250         
 104 65 90 29 31
    113 55 100 29
    108 88 117 36
  
500         
 115 70 93 27 31
    105 60 104 33
    112 68 109 33
  
750         
 110 45 141 43 40*
    101# 46 118 39
    107# 47 127 39
  
1000         
 128r 29 114 30 40
    114 24 127 37
    109 24 136 42
Positive Control Methyl Methane Sulfonate 
5         
 81 26 497 205 278*
    79 30 732 310
    62 28 596 320

Nonactivation Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 115 93 218 63 54
    107 103 153 48
    118 129 166 47
    83 75 147 59
Test Chemical 1,2-Dichloropropane (propylene dichloride) 
100         
 141r 103 141 33 52
    118 82 195 55
    108 86 161 50
  
200         
 104 98 119 38 35
    102 80 92 30
    120 89 136 38
  
400         
 115 84 151 44 41
    119 85 138 39
    123r 91 92 25
  
600         
 134r 64 167 42 69
    118 69 232 66
    68 41 149 73
  
800         
 102 47 158 52 52
    116 52 150 43
    98 66 179 61
  
1000         
 77r 31 129 56
    123r 38 143 39
    128r 64 127 33
Positive Control Methyl Methane Sulfonate 
5         
 103 61 1368 444 495*
    70 55 998 473
    70 51 1197 567

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 98 88 156 53 51
    92 103 152 55
    80 113 121 50
    78 95 107 46
Test Chemical 1,2-Dichloropropane (propylene dichloride) 
3.13      
 101 101 167 55 53
    83 78 144 58
    108 111 148 46
  
6.25      
 108 98 147 45 49
    92 97 130 47
    109 102 173 53
  
12.5       
 101 85 155 51 59
    89 85 163 61
    86 83 162 63
  
25         
 101 87 227 75 72
    103 87 234 75
    91 83 183 67
  
50         
 91 60 346 127 116*
    112 71 361 107
    110 63 381 115
  
100         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 69 65 434 210 234*
    86 53 675 261
    95 60 656 230

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 112 79 116 35 37
   
    104 100 125 40
    107 121 113 35
Test Chemical 1,2-Dichloropropane (propylene dichloride) 
10         
 114 91 211 62 60*
    109 94 187 57
    102 40 185 61
  
20         
 116 71 230 66 62*
    105 62 214 68
    115 94 181 53
  
30         
 110 52 353 107 85*
    93 51 177 64
  
40         
 99 53 216 73 105*
    117 52 217 62
    68 25 372 181
  
60         
 96 15 470 163 130*
    88 18 320 122
    114 25 363 106
  
80         
 379*
    47 4 709 505
    58 8 439 253
  
120         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 99 23 916 308 344*
    71 22 872 408
    73 19 690 316

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination