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Study Information
Study Attribute Value
CEBS Accession Number: 002-01048-0011-0000-7
Chemical Name: 1,2-Dichloropropane (propylene dichloride)
CASRN: 78-87-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 999130
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87# 108 131 50 63
    62# 105 135 72
    65# 90 129 66
    63# 98 122 65
Test Chemical 1,2-Dichloropropane (propylene dichloride) 
62.5       
 77 104 123 53 58
    72 110 136 63
  
125         
 79 119 105 44 44
    77 129 100 43
  
250         
 74 104 134 61 54
    85 100 122 48
  
500         
 75 77 124 55 50
    98 92 134 46
  
1000         
 82 16 125 51 92
    53 6 212 133
  
2000         
   
Positive Control Methyl Methane Sulfonate 
15         
 27 18 275 344 416*
    16 13 239 488

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 81 106 38 16 16
    52 101 29 19
    70 101 31 15
    77 93 32 14
Test Chemical 1,2-Dichloropropane (propylene dichloride) 
200         
 91 115 34 12 11
    85 90 25 10
  
400         
 80 86 56 23 24
    68 70 52 26
  
600         
 84 57 33 13 17
    115 53 70 20
  
800         
 89 42 38 14 19
    80 39 57 24
  
1000         
 61 14 50 27 26
    60# 10 44 24
  
1200         
   
Positive Control Methyl Methane Sulfonate 
15         
 29 25 127 149 158*
    37 27 183 167

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 106 132 77 24 27
    94 117 69 25
    74 72 79 36
    82 78 59 24
Test Chemical 1,2-Dichloropropane (propylene dichloride) 
20         
 72 64 71 33 50*
    61 43 121 66
  
30         
 44 13 219 167 148*
    42 16 165 130
  
40         
 34 5 216 210 204*
    35 5 210 198
  
50         
 32 3 125 131 184*
    30 3 215 238
  
60         
 28 3 99 120 136*
    31 3 143 152
  
70         
   
Positive Control 3-Methylcholanthrene 
2.5       
 52 43 445 283 263*
    46 44 335 242

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 73 91 126 58 57
    73 101 98 45
    68 111 124 61
    58 98 111 64
Test Chemical 1,2-Dichloropropane (propylene dichloride) 
18         
 43 43 119 92 80
    72 88 147 69
  
24         
 64 36 121 64 72
    59 47 143 80
  
30         
 50 35 160 107 102*
    48 33 139 96
  
36         
 28 14 185 224 193*
    42 17 203 162
  
42         
 26 8 181 237 239*
    24 5 174 242
  
48         
 20 3 209 345 323*
    20 3 179 301
Positive Control 3-Methylcholanthrene 
2.5       
 32 18 330 347 349*
    20 14 214 351

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination