Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01058-0011-0000-8
Chemical Name:	1,2-Epoxybutane
CASRN:	106-88-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	119097
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	75	105	61	27	30
			85	95	71	28
			76	122	51	22
			66	78	86	44
Test Chemical	1,2-Epoxybutane	50	60	88	80	44	38
			86	95	83	32	38
		100	85	92	75	29	36
			59	71	77	43	36
		200	87	79	120	46	41
			85	83	90	35	41
		400	82#	80	267	109	121*
			21	28	82	132	121*
Positive Control	Ethyl Methane Sulfonate	250	74	66	366	165	173*
Positive Control			58	54	317	181	173*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	50	87	121	80	69
			65	116	88	45
			68	99	124	61
			60	98	163	91
Test Chemical	1,2-Epoxybutane	50	62	105	112	61	65
			65	97	135	70	65
		100	43	73	103	80	65
			61	115	90	49	65
		200	68	102	156	77	83
			64	106	171	89	83
		400	69	102	230	111	109*
			58	84	188	108	109*
		800	48	46	775	544	492*
			34	49	453	440	492*
Positive Control	Ethyl Methane Sulfonate	250	48	64	497	349	311*
Positive Control			54	76	443	273	311*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	81	82	65	27	34
			82	95	96	39
			86	109	68	26
			86	114	117	46
Test Chemical	1,2-Epoxybutane	50	61	91	32	17	21
			80	108	59	25	21
		100	76	103	60	26	38
			66	94	97	49	38
		200	26	39	48	62	57*
			54	64	86	53	57*
		400	43	50	107	83	65*
			69	67	98	47	65*
		800	28	21	160	194	348*
			13	8	193	501	348*
		1600

Positive Control	3-Methylcholanthrene	2.5	40	25	442	370	368*
Positive Control			43	19	468	366	368*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	95	87	206	73	68
			95	93	185	65
			98	106	229	78
			108	114	181	56
Test Chemical	1,2-Epoxybutane	50	97	76	161	55	48
			86	63	107	41	48
		100	76	78	129	57	55
			87	73	137	53	55
		200	86	58	205	79	79
			87	71	205	79	79
		400	81	48	380	157	161*
			71	46	351	166	161*
		800	48	10	655	452	559*
			34	8	672	665	559*
		1600

Positive Control	3-Methylcholanthrene	2.5	83	61	584	235	303*
Positive Control			70	38	775	371	303*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination