Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01058-0012-0000-9
Chemical Name:	1,2-Epoxybutane
CASRN:	106-88-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G18246
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	80	108	68	28	27
			83	91	59	24
			85	102	62	24
			97#	100	88	30
Test Chemical	1,2-Epoxybutane	0.0313	76	110	78	34	44*
			68	93	108	53	44*
		0.0625	99#	104	97	33	40
			79	92	110	47	40
		0.125	79	65	129	55	45*
			85	90	88	35	45*
		0.25	88	78	208	79	81*
			78	71	192	83	81*
		0.5	67	21	535	265	261*
			81	25	625	257	261*
Positive Control	Ethyl Methane Sulfonate	500	46#	27	1040	758	787*
Positive Control			38	23	922	816	787*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	98	106	116	40	36
			87	87	108	41
			112#	111	105	31
			96	97	88	31
Test Chemical	1,2-Epoxybutane	0.0313	94	101	87	31	30
			95	95	84	29	30
		0.0625	91	89	94	34	31
			98	102	79	27	31
		0.125	103	100	170	55	58*
			78	94	142	61	58*
		0.25	78	78	218	94	90*
			93	82	243	87	90*
		0.5	72	24	761	353	366*
			56	20	635	379	366*
		1

Positive Control	Ethyl Methane Sulfonate	500	61	35	1434	781	695*
Positive Control			71	46	1288	609	695*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	91	89	210	77	71
			86	95	188	73
			85	91	180	71
			84	125	166	66
Test Chemical	1,2-Epoxybutane	0.0156	65#	75	205	105	101
			72	102	208	97	101
		0.0313	74	83	284	127	127*
			92	90	346	126	127*
		0.0625	88	30	452	172	189*
			60	30	371	206	189*
		0.125	61	21	586	319	258*
			86#	22	507	197	258*
		0.25	60	14	659	367	422*
			42	6	600	476	422*
		0.5

Positive Control	3-Methylcholanthrene	5	39#	13	616.5	534	593*
Positive Control			34	9	658	651	593*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	92	101	124	45	45
Vehicle Control			108	99	147	45	45
Test Chemical	1,2-Epoxybutane	0.0313	109	72	160	49	52
			102	77	165	54	52
		0.0625	77	40	189	82	84*
			94	43	240	86	84*
		0.125	86	31	224	87	95*
			84	36	257	103	95*
		0.25	72	21	459	212	201*
			72	23	408	190	201*
		0.5	36	4	585	542	518*
			31	4	455	495	518*
Positive Control	3-Methylcholanthrene	5	58	21	721	411	425*
Positive Control			53	19	699	438	425*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination