Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01058-0013-0000-0
Chemical Name:	1,2-Epoxybutane
CASRN:	106-88-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G71732
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	101	89	35	47
Vehicle Control			83	99	149	60	47
Test Chemical	1,2-Epoxybutane	0.174	93	88	221	79	83*
			88	86	231	88	83*
		0.204	79	65	288	121	110*
			94	79	276	98	110*
		0.24	85	67	286	112	115*
			82	67	290	119	115*
		0.283	84	60	358	142	145*
			82	65	365	148	145*
		0.333	84	55	406	161	166*
			74	48	378	171	166*
		0.392	65	38	436	223	230*
			64	40	454	236	230*
		0.461	63	30	496	263	262*
			58	28	455	261	262*
		0.542	41	15	483	394	363*
			45	16	445	332	363*
		0.638	33	8	396	404	441*
			29	7	418	478	441*
		0.75	22	4	331	505	511*
			20	4	310	517	511*
Positive Control	Ethyl Methane Sulfonate	500	54	40	904	556	584*
Positive Control			49	40	897	612	584*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96	101	316	110	111
Vehicle Control			90	99	304	112	111
Test Chemical	1,2-Epoxybutane	0.066	66	40	451	228	238*
			66	40	489	247	238*
		0.077	54	29	612	379	347*
			60	32	561	314	347*
		0.091	41	16	575	467	424*
			45	15	514	381	424*
		0.107	33	8	499	509	535*
			29	7	479	560	535*
		0.126	23	5	428	611	580*
			26	4	433	548	580*
		0.148	21	3	321	518

		0.174

Positive Control	3-Methylcholanthrene	5	86	64	540	208	239*
Positive Control			66	52	538	270	239*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	112	106	175	52	60
			117	109	193	55
			109	103	208	63
			89	83	191	71
Test Chemical	1,2-Epoxybutane	0.037	94	77	274	97	98*
			84	77	250	99	98*
		0.044	96	88	260	90	98*
			106	86	337	106	98*
		0.051	94	80	361	128	106*
			94	78	236	84	106*
		0.06	108	93	266	82	81
			87	76	208	80	81
		0.071	93	66	351	126	119*
			96	71	322	112	119*
		0.084	103	69	405	132	146*
			77	54	374	161	146*
		0.098	80	49	450	187	186*
			84	49	468	185	186*
		0.116	78	34	542	233	229*
			74	32	497	225	229*
		0.136	55	13	609	372	357*
			65	17	667	341	357*
		0.16	43	6	619	485	510*
			36	5	575	535	510*
Positive Control	3-Methylcholanthrene	5	78	45	709	302	321*
			73	42	719	328
			68	32	680	333

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination