Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01061-0006-0000-6
Chemical Name:	1,2-Epoxyhexadecane
CASRN:	7320-37-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	521012
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	80	78	20	8	13
			99	108	40	13
			98	114	44	15
			99#	101	51	17
Test Chemical	1,2-Epoxyhexadecane	5	100	99	23	8	8
			91	98	24	9	8
		10	107#	97	46.5	15	13
			110	97	38	12	13
		20	86	21	49	19	18
			99	10	50	17	18
		40

Positive Control	Methyl Methane Sulfonate	15	40	26	72	60	50*
Positive Control			59	28	70	40	50*

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	52	100	72	46	45
			41r	77	63	51
			62#	100	81	44
			39r	85	86	74
Test Chemical	1,2-Epoxyhexadecane	15	57#	88	67.5	40	41
			57	84	73	43	41
		20	37	69	66	60	57
			48	70	78	55	57
		25	52	85	76	48	66
			39	61	97	83	66
		30	57#	83	73.5	43	43
			49	83	63	43	43
		35	34	73	67	65	62
			42	74	75	60	62
Positive Control	Methyl Methane Sulfonate	15	37	60	171	156	147*
Positive Control			37	62	154	139	147*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	81	97	114	47	42
			78	104	108	46
			68	95	69	34
			75	104	90	40
Test Chemical	1,2-Epoxyhexadecane	6	75	101	103	46	42
			69	98	78	37	42
		12	73	110	108	50	50
			70	106	107	51	50
		18	66	49	133	67	76*
			51	44	128	84	76*
		24	16	5	286	596
			24r	6	316	442
		30

Positive Control	Methyl Methane Sulfonate	15	40	35	145	120	124*
Positive Control			38	39	146	128	124*

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	64	99	115	60	48
			67	97	100	50
			72	94	88	41
			83	111	104	42
Test Chemical	1,2-Epoxyhexadecane	6	82	90	95	39	37
			73	92	79	36	37
		12	75	73	100	45	42
			66	76	78	40	42
		18	38	11	173	152	163*
			37	13	190	174	163*
		24	7r	1	49	239
			10r	2	67	231
		30	1r	0	8	533
			2r	0	26	400
		36

Positive Control	Methyl Methane Sulfonate	15	51	37	184	120	123*
			40	35	150	126	123*
	Methyl Methane Sulfonate	250	58	105	225	128	134*
			50	107	209	141	134*

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	106	96	215	68	58
			110	102	167	51
			104#	96	179	58
			103	106	172	56
Test Chemical	1,2-Epoxyhexadecane	2	102	116	145	48	44
			100	96	120	40	44
		10	99#	99	156	53	47
			89	122	112	42	47
		18	101	99	74	24	28
			90	103	87	32	28
		26	89#	38	96	36	36
			98	37	108	37	36
		34

Positive Control	3-Methylcholanthrene	2.5	80#	77	520.5	216	237*
Positive Control			76	84	590	258	237*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	91#	97	45	17	20
			79	94	61	26
			91	110	65	24
			109#	99	51	16
Test Chemical	1,2-Epoxyhexadecane	6	100	100	77	26	26
			90	103	72	27	26
		12	60	78	69	38	40*
			72	88	90	42	40*
		18	64	66	61	32	30
			69	71	57	28	30
		24	46	50	48	35	37*
			52	55	61	39	37*
		30	60	40	61	34	41*
			41	30	60	48	41*
		36

Positive Control	3-Methylcholanthrene	2.5	29	7	163	191	227*
Positive Control			18	5	142	263	227*

Induced S9 Trial: 3 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	80	107	128	54	55
			77	100	122	53
			65	93	114	59
			74	100	119	54
Test Chemical	1,2-Epoxyhexadecane	18	92	120	105	38	42
			82	102	112	46	42
		24	70	91	100	48	45
			87	99	110	42	45
		30	62	88	93	50	42
			71	73	75	35	42
		36	72	73	79	37	38
			82	48	97	40	38
		42	65	48	115	59	61
			69	26	130	63	61
Positive Control	3-Methylcholanthrene	2.5	43	31	471	362	371*
Positive Control			42	28	480	379	371*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination