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Study Information
Study Attribute Value
CEBS Accession Number: 002-01061-0006-0000-6
Chemical Name: 1,2-Epoxyhexadecane
CASRN: 7320-37-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 521012
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 80 78 20 8 13
    99 108 40 13
    98 114 44 15
    99# 101 51 17
Test Chemical 1,2-Epoxyhexadecane 
5         
 100 99 23 8 8
    91 98 24 9
  
10         
 107# 97 46.5 15 13
    110 97 38 12
  
20         
 86 21 49 19 18
    99 10 50 17
  
40         
   
Positive Control Methyl Methane Sulfonate 
15         
 40 26 72 60 50*
    59 28 70 40

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 52 100 72 46 45
    41r 77 63 51
    62# 100 81 44
    39r 85 86 74
Test Chemical 1,2-Epoxyhexadecane 
15         
 57# 88 67.5 40 41
    57 84 73 43
  
20         
 37 69 66 60 57
    48 70 78 55
  
25         
 52 85 76 48 66
    39 61 97 83
  
30         
 57# 83 73.5 43 43
    49 83 63 43
  
35         
 34 73 67 65 62
    42 74 75 60
Positive Control Methyl Methane Sulfonate 
15         
 37 60 171 156 147*
    37 62 154 139

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 81 97 114 47 42
    78 104 108 46
    68 95 69 34
    75 104 90 40
Test Chemical 1,2-Epoxyhexadecane 
6         
 75 101 103 46 42
    69 98 78 37
  
12         
 73 110 108 50 50
    70 106 107 51
  
18         
 66 49 133 67 76*
    51 44 128 84
  
24         
 16 5 286 596
    24r 6 316 442
  
30         
   
Positive Control Methyl Methane Sulfonate 
15         
 40 35 145 120 124*
    38 39 146 128

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 64 99 115 60 48
    67 97 100 50
    72 94 88 41
    83 111 104 42
Test Chemical 1,2-Epoxyhexadecane 
6         
 82 90 95 39 37
    73 92 79 36
  
12         
 75 73 100 45 42
    66 76 78 40
  
18         
 38 11 173 152 163*
    37 13 190 174
  
24         
 7r 1 49 239
    10r 2 67 231
  
30         
 1r 0 8 533
    2r 0 26 400
  
36         
   
Positive Control Methyl Methane Sulfonate 
15         
 51 37 184 120 123*
    40 35 150 126
Methyl Methane Sulfonate 
250         
 58 105 225 128 134*
    50 107 209 141

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 106 96 215 68 58
    110 102 167 51
    104# 96 179 58
    103 106 172 56
Test Chemical 1,2-Epoxyhexadecane 
2         
 102 116 145 48 44
    100 96 120 40
  
10         
 99# 99 156 53 47
    89 122 112 42
  
18         
 101 99 74 24 28
    90 103 87 32
  
26         
 89# 38 96 36 36
    98 37 108 37
  
34         
   
Positive Control 3-Methylcholanthrene 
2.5       
 80# 77 520.5 216 237*
    76 84 590 258

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 91# 97 45 17 20
    79 94 61 26
    91 110 65 24
    109# 99 51 16
Test Chemical 1,2-Epoxyhexadecane 
6         
 100 100 77 26 26
    90 103 72 27
  
12         
 60 78 69 38 40*
    72 88 90 42
  
18         
 64 66 61 32 30
    69 71 57 28
  
24         
 46 50 48 35 37*
    52 55 61 39
  
30         
 60 40 61 34 41*
    41 30 60 48
  
36         
   
Positive Control 3-Methylcholanthrene 
2.5       
 29 7 163 191 227*
    18 5 142 263

Induced S9 Trial: 3 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 80 107 128 54 55
    77 100 122 53
    65 93 114 59
    74 100 119 54
Test Chemical 1,2-Epoxyhexadecane 
18         
 92 120 105 38 42
    82 102 112 46
  
24         
 70 91 100 48 45
    87 99 110 42
  
30         
 62 88 93 50 42
    71 73 75 35
  
36         
 72 73 79 37 38
    82 48 97 40
  
42         
 65 48 115 59 61
    69 26 130 63
Positive Control 3-Methylcholanthrene 
2.5       
 43 31 471 362 371*
    42 28 480 379

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination