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Study Information
Study Attribute Value
CEBS Accession Number: 002-01070-0012-0000-3
Chemical Name: 1,3-Butadiene
CASRN: 106-99-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 614566
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Air 
 0         
 107 115 51 16 19
    87 91 46 18
    99 99 72 24
    84 96 43 17
Test Chemical 1,3-Butadiene 
2.5       
 86 112 61 24 22
    107 111 68 21
  
5         
 89 91 50 19 23
    74 92 60 27
  
10         
 82 104 59 24 21
    95 92 53 19
  
20         
 98 95 63 22 25
    101 91 85 28
  
30         
 94 89 47 17 20
    91 90 61 22
Positive Control Methyl Methane Sulfonate 
15         
 37 20 158 141 135*
    41 20 159 128

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Air 
 0         
 104 92 93 30 21
    89 108 43 16
    101 103 56 18
    99 96 61 21
Test Chemical 1,3-Butadiene 
2.5       
 112 100 57 17 14
    112 101 37 11
  
5         
 97 97 54 18 23
    86 90 72 28
  
10         
 94 92 72 25 28
    98 94 91 31
  
20         
 93 76 42 15 19
    112 88 75 22
  
30         
 98 79 72 25 22
    92 74 53 19
Positive Control Methyl Methane Sulfonate 
15         
 41 31 225 184 189*
    33 24 191 194

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Air 
 0         
 88 97 78 30 24
    70 79 62 30
    83 91 51 20
    107 133 52 16
Test Chemical 1,3-Butadiene 
2.5       
 76 49 78 34 29
    75 87 55 25
  
5         
 91 50 82 30 29
    97 58 79 27
  
10         
 76 62 97 43 32
    82 91 54 22
  
20         
 81 72 72 30 27
    66 62 49 25
  
30         
 80# 69 80 33 34
    90 65 93 35
Positive Control 3-Methylcholanthrene 
2.5       
 51 28 355 234 236*
    48 42 340 239

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Air 
 0         
 81 98 39 16 19
    87 101 35 13
    93 97 65 23
    88 104 60 23
Test Chemical 1,3-Butadiene 
2.5       
 98 86 43 15 17
    71 80 40 19
  
5         
 64 84 61 32 27
    92 85 62 22
  
10         
 82 99 40 16 29
    78 87 98 42
  
20         
 73 75 65 30 28
    81 80 66 27
  
30         
 76 76 57 25 31
    68 75 75 37
Positive Control 3-Methylcholanthrene 
2.5       
 40 24 255 213 227*
    39 30 281 240

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination