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Study Information
Study Attribute Value
CEBS Accession Number: 002-01091-0010-0000-4
Chemical Name: 1,4-Dichlorobenzene (p-dichlorobenzene)
CASRN: 106-46-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 465704
Study Result: Equivocal


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 73 118 82 37 34
    70 91 71 34
    83 101 98 39
    84 91 62 25
Test Chemical 1,4-Dichlorobenzene (p-dichlorobenzene) 
6.25      
 82 91 90 36 44
    77 108 118 51
  
12.5       
 77 82 153 67 62*
    81 89 140 58
  
25         
 71 75 80 37 42
    88 86 122 46
  
50         
 79 53 96 41 42
    95 50 126 44
  
100         
   
Positive Control Methyl Methane Sulfonate 
15         
 45 32 342 255 286*
    37 31 347 317

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 97 88 35 38
    91 115 108 40
    99 95 97 33
    80# 93 109 46
Test Chemical 1,4-Dichlorobenzene (p-dichlorobenzene) 
30         
 87 72 107 41 37
    86 84 83 32
  
40         
 94 60 158 56
    127r 71 139 36
  
50         
 110 59 138 42
    133r 60 132 33
  
60         
 112 41 163 49 46
    98 43 128 43
  
70         
 102 18 250 82 90*
    85 10 248 97
  
80         
    106 20 226 71
  
90         
   
Positive Control Methyl Methane Sulfonate 
15         
 42 24 486 386 364*
    44 21 456 343

Nonactivation Trial: 3 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 96 119 46 40
    77 100 92 40
    93 99 105 38
    85 104 93 36
Test Chemical 1,4-Dichlorobenzene (p-dichlorobenzene) 
70         
 67 79 96 48 43
    73 82 84 39
  
75         
 90 63 119 44 39
    80 54 82 34
  
80         
 61 60 88 48 43
    62 63 71 38
  
85         
 69 39 74 36 37
    85 76 97 38
  
90         
 75 57 72 32 33
    90 48 93 35
Positive Control Methyl Methane Sulfonate 
15         
 24 20 191 269 245*
    27 20 178 221

Nonactivation Trial: 4 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 75 97 73 33 36
    88 101 77 29
    88 100 113 43
    79 102 90 38
Test Chemical 1,4-Dichlorobenzene (p-dichlorobenzene) 
55         
 89 80 107 40 40
    95 82 115 40
  
65         
 67 77 66 33 36
    73 81 86 39
  
75         
 66 67 83 42
  
85         
 87 49 123 47 43
    73 47 83 38
  
95         
 76 16 144 63 53
    86 15 109 42
  
105         
   
Positive Control Methyl Methane Sulfonate 
15         
 26 23 315 406 362*
    25 29 236 317

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 73 105 154 71 69
    67 87 167 83
    91 88 148 54
    76 121 159 70
Test Chemical 1,4-Dichlorobenzene (p-dichlorobenzene) 
60         
 79 59 187 79 81
    72 64 180 84
  
70         
 80 61 187 78 76
    58 46 129 74
  
80         
 61 38 177 96 98
    46 39 136 99
  
90         
 47 21 115 82 87
    54 21 147 91
  
100         
 79 21 218 92 88
    58 18 147 84
  
110         
   
Positive Control 3-Methylcholanthrene 
2.5       
 66 50 561 285 293*
    67 64 598 300

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 70 96 86 41 35
    68 89 73 36
    86 117 67 26
    63# 98 67 35
Test Chemical 1,4-Dichlorobenzene (p-dichlorobenzene) 
55         
 69 55 67 32 33
    67 51 67 33
  
65         
 55 39 78 48 56*
    58 35 112 64
  
75         
 62 28 85 46 44
    57 30 74 43
  
85         
 70 23 83 40 41
    73 21 92 42
  
95         
 64 12 140 73 88*
    50 10 155 104
  
105         
   
Positive Control 3-Methylcholanthrene 
2.5       
 41 33 466 384 363*
    48 33 498 343

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 97 188 72 73
    81 100 175 72
    67# 103 161 80
    80# 100 166.5 70
Test Chemical 1,4-Dichlorobenzene (p-dichlorobenzene) 
80         
 69# 42 211.5 102 96
    69 46 188 91
  
85         
 73# 27 247 112 108
    69 26 214 104
  
90         
 95# 37 175.5 62 87
    92# 26 306 111
  
95         
 87# 27 241.5 92 100
    67 19 217 108
  
100         
 58 19 229 133 119*
    77 17 245 106
  
105         
   
Positive Control 3-Methylcholanthrene 
2.5       
 71 64 652 308 356*
    52 44 627 405

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination