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Study Information
Study Attribute Value
CEBS Accession Number: 002-01093-0013-0000-9
Chemical Name: 1,4-Dioxane
CASRN: 123-91-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 120802
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 87 101 183 70 69
    80 98 160 66
    85# 101 178 70
Test Chemical 1,4-Dioxane 
312.5       
 90 106 190 70 64
    88# 122 150 57
  
625         
 79# 87 196.5 83 74
    101 103 195 64
  
1250         
 83# 102 190.5 76 82
    92 95 239 87
  
2500         
 66# 96 141 72 73
    91 104 201 74
  
5000         
 77 114 186 80 69
    87 100 151 58
Positive Control Methyl Methane Sulfonate 
15         
 42 33 466 374 366*
    42# 28 445 357

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 76 107 70 31 36
    69# 91 70.5 34
    64 104 67 35
    72 98 96 44
Test Chemical 1,4-Dioxane 
1000         
 62 99 74 40 31
    73 113 48 22
  
2000         
 73 87 115 53 41
    83 82 74 30
  
4000         
 41# 58 84 68 48
    70 90 57 27
  
5000         
 46# 57 82.5 60 59*
    46# 55 81 58
Positive Control Methyl Methane Sulfonate 
15         
 34# 29 298.5 294 294*

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 81 93 124 51 46
    86 105 138 53
    92 92 102 37
    84 109 108 43
Test Chemical 1,4-Dioxane 
1000         
 95 99 99 35 40
    107 98 144 45
  
2000         
 80 90 127 53 47
    93 109 115 41
  
3000         
 84 114 79 31 30
    86 115 76 30
  
4000         
 86 100 90 35 40
    88 116 117 44
  
5000         
 102 98 85 28 31
    94 102 98 35
Positive Control Methyl Methane Sulfonate 
15         
 51 26 396 261 236*
    52 34 331 212

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 96 96 212 73 75
    98 95 213 72
    83 89 218 87
    96 120 189 66
Test Chemical 1,4-Dioxane 
1000         
 103 104 216 70 74
    93 102 215 77
  
2000         
 95 114 209 73 71
    108 101 221 68
  
3000         
 89 95 190 71 68
    95 101 185 65
  
4000         
 78 99 158 68 66
    88 83 169 64
  
5000         
 67 82 170 85
    127r 103 230 60
Positive Control 3-Methylcholanthrene 
2.5       
 79 59 663 279 265*
    88 70 665 252

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 96 102 107 37 45
    91 94 94 35
    74 82 129 58
    86 122 130 50
Test Chemical 1,4-Dioxane 
1000         
 80 94 110 46 50
    70 89 111 53
  
2000         
 78 77 121 52 51
    78 77 116 50
  
3000         
 62 65 88 48 48
    79 68 114 48
  
4000         
 69 65 125 61 57
    60 54 96 53
  
5000         
 51 65 125 82 73*
    56 56 110 65
Positive Control 3-Methylcholanthrene 
2.5       
 59 62 279 159 151*
    77 65 331 143

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 79 113 112 47 45
    86 109 117 45
    60 66 75 42
    67 112 96 48
Test Chemical 1,4-Dioxane 
1000         
 68 85 106 52 51
    79 106 121 51
  
2000         
 67 80 118 59 56
    63 75 101 54
  
3000         
 65 79 131 67 67
    73 94 144 66
  
4000         
 80 121 109 45 51
    38 65 65 57
  
5000         
 49 57 81 56 49
    65 89 83 43
Positive Control 3-Methylcholanthrene 
2.5       
 60 38 626 349 363*
    64 42 726 377

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination