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Study Information
Study Attribute Value
CEBS Accession Number: 002-01096-0002-0000-0
Chemical Name: 1,5-Naphthalenediamine
CASRN: 2243-62-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 531218_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #:1   Activation: No Activation    Date: 07/08/1987    Harvest Time: 19.9 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 1,5-Naphthalenediamine 
150         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
175         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
200         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 100 24 0.240 15.0 11 0.110 10.0 13 0.130 9.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 8 0.320 24.0 6 0.240 20.0 2 0.080 8.0 0 0.000 0.0
Trend: -0.652 -0.460 -0.460  
Probability: 0.743 0.677 0.677  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 08/11/1987    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 7 0.070 5.0 1 0.010 1.0 6 0.060 4.0 0 0.000 0.0
Test Chemical: 1,5-Naphthalenediamine 
2         
 25 13 0.520 44.0 4 0.160 16.0 9 0.360 28.0 0 0.000 0.0 
2.5       
 25 43 1.720 68.0 6 0.240 16.0 27 1.080 56.0 10 0.400 4.0 
3         
 25 25 1.000 64.0 5 0.200 20.0 20 0.800 60.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 100 11 0.110 11.0 2 0.020 2.0 9 0.090 9.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 20 0.800 40.0 4 0.160 16.0 6 0.240 24.0 10 0.400 4.0
Trend: 7.681 3.716 7.351  
Probability: 0.000 0.000 0.000  


Trial #:2   Activation: No Activation    Date: 06/18/1987    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 1,5-Naphthalenediamine 
75         
 200 7 0.035 2.5 3 0.015 1.5 4 0.020 1.5 0 0.000 0.0 
112.5       
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0 
150         
 200 17 0.085 8.5 9 0.045 4.5 8 0.040 4.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 25 0.125 11.0 13 0.065 6.5 12 0.060 5.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 40 1.600 72.0 14 0.560 40.0 16 0.640 52.0 10 0.400 4.0
Trend: 4.301 2.934 2.870  
Probability: 0.000 0.002 0.002  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 06/18/1987    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 4 0.020 0.5 0 0.000 0.0 4 0.020 0.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 3 0.015 1.0 1 0.005 0.5 2 0.010 0.5 0 0.000 0.0
Test Chemical: 1,5-Naphthalenediamine 
1         
 200 82 0.410 27.5 30 0.150 14.0 52 0.260 19.5 0 0.000 0.0 
1.5       
 200 131 0.655 33.0 59 0.295 21.0 72 0.360 22.5 0 0.000 0.0 
2         
 200 70 0.350 21.5 19 0.095 8.5 51 0.255 15.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 16 0.080 7.0 8 0.040 3.5 8 0.040 4.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 13 0.520 40.0 4 0.160 16.0 9 0.360 28.0 0 0.000 0.0
Trend: 5.446 3.412 4.357  
Probability: 0.000 0.000 0.000