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Study Information
Study Attribute Value
CEBS Accession Number: 002-01135-0002-0000-4
Chemical Name: 1-Phenyl-2-thiourea
CASRN: 103-85-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 575473_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #:1   Activation: No Activation    Date: 01/16/1985    Harvest Time: 21 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 1-Phenyl-2-thiourea 
75         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
100         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0 
250         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
500         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.04      
 100 12 0.120 10.0 2 0.020 2.0 10 0.100 9.0 0 0.000 0.0
Mitomycin-C 
 0.063     
 25 6 0.240 20.0 5 0.200 16.0 1 0.040 4.0 0 0.000 0.0
Trend: 0.506 0.000 0.506  
Probability: 0.306 0.000 0.306  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 02/12/1985    Harvest Time: 21.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 1-Phenyl-2-thiourea 
3500         
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0 
4000         
 100 11 0.110 9.0 5 0.050 5.0 6 0.060 5.0 0 0.000 0.0 
4500         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
5000         
 100 13 0.130 11.0 6 0.060 6.0 7 0.070 6.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
6.25      
 100 10 0.100 8.0 3 0.030 3.0 7 0.070 5.0 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 21 0.840 48.0 10 0.400 32.0 11 0.440 40.0 0 0.000 0.0
Trend: 2.496 1.906 1.835  
Probability: 0.006 0.028 0.033  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 01/24/1985    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: 1-Phenyl-2-thiourea 
3000         
 100 34 0.340 15.0 17 0.170 10.0 17 0.170 8.0 0 0.000 0.0 
3500         
 100 35 0.350 18.0 9 0.090 7.0 16 0.160 13.0 10 0.100 1.0 
4000         
 15 7 0.467 33.0 4 0.267 27.0 3 0.200 13.0 0 0.000 0.0 
5000         
 100 7 0.070 5.0 0 0.000 0.0 7 0.070 5.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 100 22 0.220 17.0 6 0.060 5.0 16 0.160 12.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 64 2.560 100.0 20 0.800 68.0 44 1.760 88.0 0 0.000 0.0
Trend: 0.373 -0.436 0.684  
Probability: 0.355 0.668 0.247  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 02/27/1985    Harvest Time: 21.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 50 1 0.020 2.0 0 0.000 0.0 1 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 50 2 0.040 4.0 1 0.020 2.0 1 0.020 2.0 0 0.000 0.0
Test Chemical: 1-Phenyl-2-thiourea 
4000         
 50 2 0.040 4.0 0 0.000 0.0 2 0.040 4.0 0 0.000 0.0 
4500         
 50 24 0.480 28.0 8 0.160 10.0 16 0.320 26.0 0 0.000 0.0 
5000         
 50 31 0.620 30.0 15 0.300 18.0 16 0.320 26.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
6.25      
 50 5 0.100 10.0 1 0.020 2.0 4 0.080 8.0 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 18 0.720 44.0 6 0.240 24.0 12 0.480 32.0 0 0.000 0.0
Trend: 4.374 3.478 4.254  
Probability: 0.000 0.000 0.000