Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01135-0007-0000-9
Chemical Name:	1-Phenyl-2-thiourea
CASRN:	103-85-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	688781
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	86	98	45	66
			79	106	193	82
			70	107	118	56
			64	101	157	82
Test Chemical	1-Phenyl-2-thiourea	62.5	70	79	120	57	70
			78	73	194	83	70
		125	81	44	177	73	73
			75	48	166	73	73
		250	51	33	164	108	97
			60	32	154	86	97
		500

Positive Control	Methyl Methane Sulfonate	15	31	19	295	322	295*
Positive Control			33	23	265	268	295*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	76	119	192	84	86
			72	114	153	71
			71	86	231	108
			80#	80	198	83
Test Chemical	1-Phenyl-2-thiourea	50	77	100	222	96	84
			87	94	185	71	84
		125	75	101	180	80	82
			90	79	226	84	82
		200	98	82	288	98	92
			86	76	225	87	92
		275	93	52	285	102	109
			84	64	291	115	109
		350	58	24	300	173	192*
			54	35	343	210	192*
		425

Positive Control	Methyl Methane Sulfonate	15	30	25	194	218	181*
Positive Control			41	27	179	144	181*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	60	92	87	49	53
			56	82	135	80
			69	105	95	46
			64	121	69	36
Test Chemical	1-Phenyl-2-thiourea	300	69	24	186	90	103*
			57	23	199	117	103*
		325	58	16	187	107	107*
			66	19	212	107	107*
		350	50	12	187	126	130*
			46	15	184	134	130*
		375	106	31	186	58	95*
			68	11	268	132	95*
		400	37	4	218	197	130*
			70	30	130	62	130*
		425

Positive Control	Methyl Methane Sulfonate	15	29	18	200	229	252*
Positive Control			27	16	226	276	252*

Nonactivation Trial: 4 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	103	124	56	51
			87	96	147	57
			87	101	102	39
Test Chemical	1-Phenyl-2-thiourea	200	95	91	163	57	61
			81	67	156	64	61
		250	77	79	156	67	60
			88	78	140	53	60
		300	77	69	144	63	62
			85	69	155	61	62
		350	83	58	160	65	66
			97	62	195	67	66
		400	100#	45	263	88	77
			97	50	193	67	77
Positive Control	Methyl Methane Sulfonate	15	44	29	334	255	268*
Positive Control			38	29	316	281	268*

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	64	95	130	68	80
			64#	107	170	88
			36r	85	104	97
			55#	97	136.5	83
Test Chemical	1-Phenyl-2-thiourea	150	82	93	134	54	53
			89#	78	135	51	53
		225	100	92	146	49	42
			85	94	89	35	42
		300	72	84	85	40	41
			70	71	88	42	41
		375	66	57	90	45	44
			88	69	112	42	44
		450	68#	38	114	56	60
			77	36	150	65	60
		525

Positive Control	3-Methylcholanthrene	2.5	34	26	560	552	584*
Positive Control			36	22	671	616	584*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69#	97	66	32	22
			57	84	35	20
			62	119	23	12
Test Chemical	1-Phenyl-2-thiourea	225	73#	103	67.5	31	35
			63	74	75	39	35
		300	69#	44	53	26	29
			77	93	74	32	29
		375	79	82	62	26	30
			74	69	75	34	30
		450	76	59	107	47	51*
			76	56	127	55	51*
		525	52	15	86	56	69*
			56	12	137	82	69*
Positive Control	3-Methylcholanthrene	2.5	33#	22	268.5	274	273*
Positive Control			32	23	261	272	273*

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	55	118	47	29	28
			93	102	71	25
			64	72	60	31
			69	108	54	26
Test Chemical	1-Phenyl-2-thiourea	150	103	84	90	29	37
			86	67	116	45	37
		225	69	61	38	18	19
			86	62	50	19	19
		300	94	69	66	23	27
			61	56	56	31	27
		375	63	38	42	22	22
			63	53	43	23	22
		450	63	33	37	20	26
			41	16	41	33	26
		525

Positive Control	3-Methylcholanthrene	2.5	42	33	416	333	305*
Positive Control			57	42	471	278	305*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination