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Study Information
Study Attribute Value
CEBS Accession Number: 002-01135-0007-0000-9
Chemical Name: 1-Phenyl-2-thiourea
CASRN: 103-85-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 688781
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 86 98 45 66
    79 106 193 82
    70 107 118 56
    64 101 157 82
Test Chemical 1-Phenyl-2-thiourea 
62.5       
 70 79 120 57 70
    78 73 194 83
  
125         
 81 44 177 73 73
    75 48 166 73
  
250         
 51 33 164 108 97
    60 32 154 86
  
500         
   
Positive Control Methyl Methane Sulfonate 
15         
 31 19 295 322 295*
    33 23 265 268

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 76 119 192 84 86
    72 114 153 71
    71 86 231 108
    80# 80 198 83
Test Chemical 1-Phenyl-2-thiourea 
50         
 77 100 222 96 84
    87 94 185 71
  
125         
 75 101 180 80 82
    90 79 226 84
  
200         
 98 82 288 98 92
    86 76 225 87
  
275         
 93 52 285 102 109
    84 64 291 115
  
350         
 58 24 300 173 192*
    54 35 343 210
  
425         
   
Positive Control Methyl Methane Sulfonate 
15         
 30 25 194 218 181*
    41 27 179 144

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 60 92 87 49 53
    56 82 135 80
    69 105 95 46
    64 121 69 36
Test Chemical 1-Phenyl-2-thiourea 
300         
 69 24 186 90 103*
    57 23 199 117
  
325         
 58 16 187 107 107*
    66 19 212 107
  
350         
 50 12 187 126 130*
    46 15 184 134
  
375         
 106 31 186 58 95*
    68 11 268 132
  
400         
 37 4 218 197 130*
    70 30 130 62
  
425         
   
Positive Control Methyl Methane Sulfonate 
15         
 29 18 200 229 252*
    27 16 226 276

Nonactivation Trial: 4 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 74 103 124 56 51
    87 96 147 57
    87 101 102 39
Test Chemical 1-Phenyl-2-thiourea 
200         
 95 91 163 57 61
    81 67 156 64
  
250         
 77 79 156 67 60
    88 78 140 53
  
300         
 77 69 144 63 62
    85 69 155 61
  
350         
 83 58 160 65 66
    97 62 195 67
  
400         
 100# 45 263 88 77
    97 50 193 67
Positive Control Methyl Methane Sulfonate 
15         
 44 29 334 255 268*
    38 29 316 281

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 64 95 130 68 80
    64# 107 170 88
    36r 85 104 97
    55# 97 136.5 83
Test Chemical 1-Phenyl-2-thiourea 
150         
 82 93 134 54 53
    89# 78 135 51
  
225         
 100 92 146 49 42
    85 94 89 35
  
300         
 72 84 85 40 41
    70 71 88 42
  
375         
 66 57 90 45 44
    88 69 112 42
  
450         
 68# 38 114 56 60
    77 36 150 65
  
525         
   
Positive Control 3-Methylcholanthrene 
2.5       
 34 26 560 552 584*
    36 22 671 616

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 69# 97 66 32 22
    57 84 35 20
    62 119 23 12
Test Chemical 1-Phenyl-2-thiourea 
225         
 73# 103 67.5 31 35
    63 74 75 39
  
300         
 69# 44 53 26 29
    77 93 74 32
  
375         
 79 82 62 26 30
    74 69 75 34
  
450         
 76 59 107 47 51*
    76 56 127 55
  
525         
 52 15 86 56 69*
    56 12 137 82
Positive Control 3-Methylcholanthrene 
2.5       
 33# 22 268.5 274 273*
    32 23 261 272

Induced S9 Trial: 3 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 55 118 47 29 28
    93 102 71 25
    64 72 60 31
    69 108 54 26
Test Chemical 1-Phenyl-2-thiourea 
150         
 103 84 90 29 37
    86 67 116 45
  
225         
 69 61 38 18 19
    86 62 50 19
  
300         
 94 69 66 23 27
    61 56 56 31
  
375         
 63 38 42 22 22
    63 53 43 23
  
450         
 63 33 37 20 26
    41 16 41 33
  
525         
   
Positive Control 3-Methylcholanthrene 
2.5       
 42 33 416 333 305*
    57 42 471 278

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination