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Study Information
Study Attribute Value
CEBS Accession Number: 002-01136-0008-0000-1
Chemical Name: 1-Phenyl-3-methyl-5-pyrazolone
CASRN: 89-25-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 216441
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 91 97 111 41 40
    102 101 120 39
    89 98 125 47
    120 104 124 34
Test Chemical 1-Phenyl-3-methyl-5-pyrazolone 
56.25      
 100 91 191 64 60
    114 111 193 57
  
112.5       
 84 85 104 41 41
    101 85 127 42
  
225         
 98 106 116 40 40
    103 108 125 41
  
450         
 127r 92 123 32
    108 85 139 43
  
900         
 91 78 128 47 42
    97 94 108 37
Positive Control Methyl Methane Sulfonate 
15         
 74 41 193 87 101*
    65 39 222 114
Methyl Methane Sulfonate 
250         
 84 53 385 153 136*
    83 52 296 120

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 74 94 65 29 35
    57 94 65 38
    62 114 67 36
    64 98 74 38
Test Chemical 1-Phenyl-3-methyl-5-pyrazolone 
100         
 59 84 55 31 33
    47 75 50 36
  
300         
 43 82 28 22 29
    44 82 49 37
  
500         
 65 110 43 22 28
    75 101 78 35
  
700         
 56 96 45 27 28
    67 107 58 29
  
900         
 61 101 27 15 22
    59 97 51 29
Positive Control Methyl Methane Sulfonate 
15         
 26 24 75 94 90*
    24 23 61 85
Methyl Methane Sulfonate 
250         
 42 73 218 172 177*
    49 73 267 183

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 95 76 35 38
    83 88 99 40
    87 108 93 36
    112 109 138 41
Test Chemical 1-Phenyl-3-methyl-5-pyrazolone 
100         
 97 82 124 42 42
    87 85 111 42
  
300         
 71 62 121 57 54
    84 64 131 52
  
500         
 87 66 128 49 55
    105 70 190 60
  
700         
 85 58 170 67 61*
    81 68 133 55
  
900         
 92 49 180 66 67*
    81 56 163 67
Positive Control 3-Methylcholanthrene 
2.5       
 60 38 558 308 297*
    76 34 654 286

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 102 146 57 60
    79 104 156 66
    85 88 148 58
    94 105 163 58
Test Chemical 1-Phenyl-3-methyl-5-pyrazolone 
100         
 82 62 163 67 65
    75 41 142 64
  
300         
 69 35 365 177 189*
    62 37 372 201
  
500         
 44 16 389 294 286*
    44 21 368 279
  
700         
 46 11 432 312 271*
    31 5 211 229
  
900         
   
Positive Control 3-Methylcholanthrene 
2.5       
 21 5 297 479 469*
    22 6 296 459

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination