Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01136-0008-0000-1
Chemical Name:	1-Phenyl-3-methyl-5-pyrazolone
CASRN:	89-25-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	216441
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	91	97	111	41	40
			102	101	120	39
			89	98	125	47
			120	104	124	34
Test Chemical	1-Phenyl-3-methyl-5-pyrazolone	56.25	100	91	191	64	60
			114	111	193	57	60
		112.5	84	85	104	41	41
			101	85	127	42	41
		225	98	106	116	40	40
			103	108	125	41	40
		450	127r	92	123	32
			108	85	139	43
		900	91	78	128	47	42
			97	94	108	37	42
Positive Control	Methyl Methane Sulfonate	15	74	41	193	87	101*
			65	39	222	114	101*
	Methyl Methane Sulfonate	250	84	53	385	153	136*
			83	52	296	120	136*

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	94	65	29	35
			57	94	65	38
			62	114	67	36
			64	98	74	38
Test Chemical	1-Phenyl-3-methyl-5-pyrazolone	100	59	84	55	31	33
			47	75	50	36	33
		300	43	82	28	22	29
			44	82	49	37	29
		500	65	110	43	22	28
			75	101	78	35	28
		700	56	96	45	27	28
			67	107	58	29	28
		900	61	101	27	15	22
			59	97	51	29	22
Positive Control	Methyl Methane Sulfonate	15	26	24	75	94	90*
			24	23	61	85	90*
	Methyl Methane Sulfonate	250	42	73	218	172	177*
			49	73	267	183	177*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	95	76	35	38
			83	88	99	40
			87	108	93	36
			112	109	138	41
Test Chemical	1-Phenyl-3-methyl-5-pyrazolone	100	97	82	124	42	42
			87	85	111	42	42
		300	71	62	121	57	54
			84	64	131	52	54
		500	87	66	128	49	55
			105	70	190	60	55
		700	85	58	170	67	61*
			81	68	133	55	61*
		900	92	49	180	66	67*
			81	56	163	67	67*
Positive Control	3-Methylcholanthrene	2.5	60	38	558	308	297*
Positive Control			76	34	654	286	297*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	102	146	57	60
			79	104	156	66
			85	88	148	58
			94	105	163	58
Test Chemical	1-Phenyl-3-methyl-5-pyrazolone	100	82	62	163	67	65
			75	41	142	64	65
		300	69	35	365	177	189*
			62	37	372	201	189*
		500	44	16	389	294	286*
			44	21	368	279	286*
		700	46	11	432	312	271*
			31	5	211	229	271*
		900

Positive Control	3-Methylcholanthrene	2.5	21	5	297	479	469*
Positive Control			22	6	296	459	469*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination