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Study Information
Study Attribute Value
CEBS Accession Number: 002-01170-0002-0000-3
Chemical Name: 2,3,4,5-Tetrachlorophenol
CASRN: 4901-51-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 563610_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Weakly Positive* 
Induced Rat Liver S9  3_S9  Negative 

Trial #:1   Activation: No Activation    Date: 07/09/1986    Harvest Time: 20 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: 2,3,4,5-Tetrachlorophenol 
29.8       
 200 4 0.020 1.5 1 0.005 0.5 3 0.015 1.0 0 0.000 0.0 
45.2       
 200 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0 0 0.000 0.0 
60.3       
 200 5 0.025 2.5 2 0.010 1.0 3 0.015 1.5 0 0.000 0.0 
90         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 71 0.355 24.0 31 0.155 14.5 30 0.150 11.5 10 0.050 0.5
Mitomycin-C 
 0.08      
 25 17 0.680 44.0 8 0.320 28.0 9 0.360 28.0 0 0.000 0.0
Trend: 0.844 1.320 0.056  
Probability: 0.199 0.093 0.478  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 07/09/1986    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 6 0.030 3.0 6 0.030 3.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0
Test Chemical: 2,3,4,5-Tetrachlorophenol 
29.8       
 200 12 0.060 5.5 8 0.040 3.5 4 0.020 2.0 0 0.000 0.0 
45.2       
 200 29 0.145 12.0 15 0.075 7.5 14 0.070 5.5 0 0.000 0.0 
60.3       
 200 47 0.235 17.0 14 0.070 6.0 33 0.165 14.0 0 0.000 0.0 
90         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 17 0.085 6.5 3 0.015 1.5 14 0.070 5.5 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 23 0.920 56.0 14 0.560 32.0 9 0.360 32.0 0 0.000 0.0
Trend: 6.059 3.287 5.962  
Probability: 0.000 0.001 0.000  


Trial #:2   Activation: No Activation    Date: 01/26/1986    Harvest Time: 20.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 16 0.080 6.0 5 0.025 2.0 11 0.055 4.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 7 0.035 3.5 2 0.010 1.0 5 0.025 2.5 0 0.000 0.0
Test Chemical: 2,3,4,5-Tetrachlorophenol 
20         
 200 5 0.025 2.5 1 0.005 0.5 4 0.020 2.0 0 0.000 0.0 
30         
 200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0 
40         
 200 10 0.050 4.5 0 0.000 0.0 10 0.050 4.5 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 40 0.200 17.5 12 0.060 5.5 28 0.140 12.5 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 11 0.440 28.0 3 0.120 12.0 8 0.320 20.0 0 0.000 0.0
Trend: 0.160 -1.320 0.787  
Probability: 0.436 0.907 0.216  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 08/20/1986    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Test Chemical: 2,3,4,5-Tetrachlorophenol 
15.1       
 100 4 0.040 3.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 
30.1       
 100 6 0.060 5.0 1 0.010 1.0 5 0.050 5.0 0 0.000 0.0 
44.9       
 100 29 0.290 21.0 4 0.040 4.0 25 0.250 17.0 0 0.000 0.0 
59.8       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 100 11 0.110 10.0 5 0.050 5.0 6 0.060 6.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 16 0.640 28.0 3 0.120 12.0 13 0.520 20.0 0 0.000 0.0
Trend: 4.424 1.741 3.884  
Probability: 0.000 0.041 0.000  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 01/28/1986    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 4 0.020 1.5 1 0.005 0.5 3 0.015 1.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 5 0.025 2.0 0 0.000 0.0 5 0.025 2.0 0 0.000 0.0
Test Chemical: 2,3,4,5-Tetrachlorophenol 
15         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
22.5       
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0 
30         
 200 5 0.025 2.5 4 0.020 2.0 1 0.005 0.5 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 26 0.130 12.0 4 0.020 2.0 22 0.110 10.5 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 9 0.360 32.0 4 0.160 16.0 5 0.200 20.0 0 0.000 0.0
Trend: 0.169 2.565 -1.613  
Probability: 0.433 0.005 0.947