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Study Information
Study Attribute Value
CEBS Accession Number: 002-01174-0001-0000-6
Chemical Name: 2,3,5,6-Tetrachloro-4-nitroanisole
CASRN: 2438-88-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 997382_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
No Activation  3  Weakly Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 09/12/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 0 0 0 0.000 0.00 0.0 N/A
Negative (Not Specified) 
0         
 50 1043 410 0.393 8.20 26.5 0.000
Test Chemical 2,3,5,6-Tetrachloro-4-nitroanisole 
1.6       
 50 1047 472 0.451 9.44 26.5 0.000 
5         
 50 1042 415 0.398 8.30 26.5 0.000 
16         
 50 1037 567 0.547 11.34 26.5 0.000 
50         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1043 636 0.610 12.72 26.5 0.000
Mitomycin-C 
 0.005     
 10 208 296 1.423 29.60 26.5 0.000
Trend: 3.224        
Probability: 0.001        

Trial #: 2    Activation: No Activation    Date: 09/25/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1038 410 0.395 8.20 27.5 0.000
Test Chemical 2,3,5,6-Tetrachloro-4-nitroanisole 
2.5       
 50 1047 474 0.453 9.48 27.5 14.616 
5         
 50 1048 443 0.423 8.86 27.5 7.018 
10         
 50 1047 504 0.481 10.08 27.5 21.870 
20         
 50 1043 565 0.542 11.30 33.0 37.144 
30         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1045 662 0.633 13.24 27.5 60.382
Mitomycin-C 
 0.005     
 10 210 341 1.624 34.10 27.5 311.101
Trend: 4.852        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 10/22/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 445 0.425 8.90 27.0 0.000
Test Chemical 2,3,5,6-Tetrachloro-4-nitroanisole 
5         
 50 1045 419 0.401 8.38 27.0 -5.572 
10         
 50 1047 445 0.425 8.90 27.0 0.096 
20         
 50 1051 487 0.463 9.74 32.0 9.126 
30         
 50 1048 689 0.657 13.78 32.0 54.831 
40         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1048 646 0.616 12.92 27.0 45.169
Mitomycin-C 
 0.005     
 10 209 263 1.258 26.30 27.0 196.354
Trend: 7.507        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 09/12/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1051 456 0.434 9.12 26.5 0.000
Test Chemical 2,3,5,6-Tetrachloro-4-nitroanisole 
1.6       
 50 1042 383 0.368 7.66 26.5 -15.283 
5         
 50 1041 430 0.413 8.60 26.5 -4.796 
16         
 50 1044 419 0.401 8.38 26.5 -7.498 
50         
 50 1034 569 0.550 11.38 26.5 26.832 
160         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1047 748 0.714 14.96 26.5 64.662
Cyclophosphamide 
 0.6       
 10 209 216 1.033 21.60 26.5 138.202
Trend: 4.098        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 09/25/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 397 0.380 7.94 25.5 0.000
Test Chemical 2,3,5,6-Tetrachloro-4-nitroanisole 
25         
 50 1045 457 0.437 9.14 25.5 15.224 
50         
 50 1020 509 0.499 10.18 31.0 31.480 
75         
 50 1038 493 0.475 9.86 31.0 25.138 
100         
 50 1035 492 0.475 9.84 31.0 25.247 
125         
 50 1038 668 0.644 13.36 31.0 69.559 
150         
 23 480 352 0.733 15.30 31.0 93.216
Positive Control Cyclophosphamide 
 0.3       
 50 1047 687 0.656 13.74 25.5 72.883
Cyclophosphamide 
 0.6       
 10 208 187 0.899 18.70 25.5 136.875
Trend: 9.465        
Probability: 0.000