COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-01174-0002-0000-7
Chemical Name: 2,3,5,6-Tetrachloro-4-nitroanisole
CASRN: 2438-88-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 997382_CA
Study Result: Equivocal

Study Result: Equivocal
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 
No Activation  3  Questionable 

Trial #:1   Activation: No Activation    Date: 10/10/1984    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,3,5,6-Tetrachloro-4-nitroanisole 
50         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
100         
 100 2 0.020 1.0 0 0.000 0.0 0 0.000 0.0 2 0.020 1.0 
150         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
200         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 20 0.200 20.0 15 0.150 15.0 5 0.050 5.0 0 0.000 0.0
Mitomycin-C 
1         
 50 25 0.500 40.0 17 0.340 28.0 7 0.140 12.0 1 0.020 1.0
Trend: -1.182 -1.601 0.000  
Probability: 0.881 0.945 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 10/10/1984    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 2,3,5,6-Tetrachloro-4-nitroanisole 
50         
 100 3 0.030 3.0 3 0.030 3.0 0 0.000 0.0 0 0.000 0.0 
100         
 100 4 0.040 3.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 
150         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
200         
 100 7 0.070 5.0 0 0.000 0.0 7 0.070 5.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 18 0.180 18.0 12 0.120 12.0 6 0.060 6.0 0 0.000 0.0
Cyclophosphamide 
50         
 50 22 0.440 30.0 14 0.280 20.0 8 0.160 14.0 0 0.000 0.0
Trend: 0.607 -1.449 2.154  
Probability: 0.272 0.926 0.016  


Trial #:2   Activation: No Activation    Date: 10/10/1984    Harvest Time: 17 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,3,5,6-Tetrachloro-4-nitroanisole 
50         
 100 7 0.070 6.0 3 0.030 3.0 3 0.030 3.0 1 0.010 1.0 
100         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
150         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
200         
 100 8 0.080 6.0 2 0.020 2.0 6 0.060 4.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 100 20 0.200 20.0 15 0.150 15.0 5 0.050 5.0 0 0.000 0.0
Mitomycin-C 
1         
 50 25 0.500 40.0 17 0.340 28.0 7 0.140 12.0 1 0.020 1.0
Trend: 0.419 -0.444 0.985  
Probability: 0.338 0.671 0.162  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 10/10/1984    Harvest Time: 19 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 2,3,5,6-Tetrachloro-4-nitroanisole 
50         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
100         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0 
150         
 100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 
200         
 100 4 0.040 3.0 1 0.010 1.0 3 0.030 2.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 18 0.180 18.0 12 0.120 12.0 6 0.060 6.0 0 0.000 0.0
Cyclophosphamide 
50         
 50 22 0.440 30.0 14 0.280 20.0 8 0.160 14.0 0 0.000 0.0
Trend: 0.612 -0.156 1.175  
Probability: 0.270 0.562 0.120  


Trial #:3   Activation: No Activation    Date: 03/13/1985    Harvest Time: 12.5 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,3,5,6-Tetrachloro-4-nitroanisole 
10         
 100 5 0.050 4.0 2 0.020 2.0 3 0.030 2.0 0 0.000 0.0 
25         
 100 5 0.050 5.0 1 0.010 1.0 4 0.040 4.0 0 0.000 0.0 
50         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
100         
 100 7 0.070 6.0 1 0.010 1.0 6 0.060 5.0 0 0.000 0.0 
150         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 19 0.190 16.0 8 0.080 7.0 11 0.110 11.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 12 0.240 22.0 8 0.160 16.0 4 0.080 8.0 0 0.000 0.0
Trend: 1.478 -0.675 2.120  
Probability: 0.070 0.750 0.017