Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01174-0002-0000-7 |
Chemical Name: | 2,3,5,6-Tetrachloro-4-nitroanisole |
CASRN: | 2438-88-2 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 997382_CA |
Study Result: | Equivocal |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Negative |
Induced Rat Liver S9 | 1_S9 | Negative |
No Activation | 2 | Negative |
Induced Rat Liver S9 | 2_S9 | Negative |
No Activation | 3 | Questionable |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,3,5,6-Tetrachloro-4-nitroanisole | 50 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
100 | 100 | 2 | 0.020 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 2 | 0.020 | 1.0 | ||
150 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
200 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.25 | 100 | 20 | 0.200 | 20.0 | 15 | 0.150 | 15.0 | 5 | 0.050 | 5.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 1 | 50 | 25 | 0.500 | 40.0 | 17 | 0.340 | 28.0 | 7 | 0.140 | 12.0 | 1 | 0.020 | 1.0 | |
Trend: | -1.182 | -1.601 | 0.000 | ||||||||||||
Probability: | 0.881 | 0.945 | 0.000 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 3 | 0.030 | 3.0 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,3,5,6-Tetrachloro-4-nitroanisole | 50 | 100 | 3 | 0.030 | 3.0 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
100 | 100 | 4 | 0.040 | 3.0 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
150 | 100 | 3 | 0.030 | 3.0 | 1 | 0.010 | 1.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
200 | 100 | 7 | 0.070 | 5.0 | 0 | 0.000 | 0.0 | 7 | 0.070 | 5.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 15 | 100 | 18 | 0.180 | 18.0 | 12 | 0.120 | 12.0 | 6 | 0.060 | 6.0 | 0 | 0.000 | 0.0 |
Cyclophosphamide | 50 | 50 | 22 | 0.440 | 30.0 | 14 | 0.280 | 20.0 | 8 | 0.160 | 14.0 | 0 | 0.000 | 0.0 | |
Trend: | 0.607 | -1.449 | 2.154 | ||||||||||||
Probability: | 0.272 | 0.926 | 0.016 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,3,5,6-Tetrachloro-4-nitroanisole | 50 | 100 | 7 | 0.070 | 6.0 | 3 | 0.030 | 3.0 | 3 | 0.030 | 3.0 | 1 | 0.010 | 1.0 |
100 | 100 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
150 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
200 | 100 | 8 | 0.080 | 6.0 | 2 | 0.020 | 2.0 | 6 | 0.060 | 4.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.25 | 100 | 20 | 0.200 | 20.0 | 15 | 0.150 | 15.0 | 5 | 0.050 | 5.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 1 | 50 | 25 | 0.500 | 40.0 | 17 | 0.340 | 28.0 | 7 | 0.140 | 12.0 | 1 | 0.020 | 1.0 | |
Trend: | 0.419 | -0.444 | 0.985 | ||||||||||||
Probability: | 0.338 | 0.671 | 0.162 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 3 | 0.030 | 3.0 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,3,5,6-Tetrachloro-4-nitroanisole | 50 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
100 | 100 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
150 | 100 | 4 | 0.040 | 4.0 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
200 | 100 | 4 | 0.040 | 3.0 | 1 | 0.010 | 1.0 | 3 | 0.030 | 2.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 15 | 100 | 18 | 0.180 | 18.0 | 12 | 0.120 | 12.0 | 6 | 0.060 | 6.0 | 0 | 0.000 | 0.0 |
Cyclophosphamide | 50 | 50 | 22 | 0.440 | 30.0 | 14 | 0.280 | 20.0 | 8 | 0.160 | 14.0 | 0 | 0.000 | 0.0 | |
Trend: | 0.612 | -0.156 | 1.175 | ||||||||||||
Probability: | 0.270 | 0.562 | 0.120 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,3,5,6-Tetrachloro-4-nitroanisole | 10 | 100 | 5 | 0.050 | 4.0 | 2 | 0.020 | 2.0 | 3 | 0.030 | 2.0 | 0 | 0.000 | 0.0 |
25 | 100 | 5 | 0.050 | 5.0 | 1 | 0.010 | 1.0 | 4 | 0.040 | 4.0 | 0 | 0.000 | 0.0 | ||
50 | 100 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 | ||
100 | 100 | 7 | 0.070 | 6.0 | 1 | 0.010 | 1.0 | 6 | 0.060 | 5.0 | 0 | 0.000 | 0.0 | ||
150 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.125 | 100 | 19 | 0.190 | 16.0 | 8 | 0.080 | 7.0 | 11 | 0.110 | 11.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.25 | 50 | 12 | 0.240 | 22.0 | 8 | 0.160 | 16.0 | 4 | 0.080 | 8.0 | 0 | 0.000 | 0.0 | |
Trend: | 1.478 | -0.675 | 2.120 | ||||||||||||
Probability: | 0.070 | 0.750 | 0.017 |