Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01174-0008-0000-3
Chemical Name:	2,3,5,6-Tetrachloro-4-nitroanisole
CASRN:	2438-88-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	665415
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	64	97	89	47	52
			69	101	98	47
			62	98	126	68
			53	104	76	48
Test Chemical	2,3,5,6-Tetrachloro-4-nitroanisole	1.25	78	110	133	57	56
			82	109	134	54	56
		2.5	67	89	120	60	64
			69	95	142	69	64
		5	83	92	125	50	49
			74	99	106	48	49
		10	64	32	127	66	65
			47	25	90	64	65
		20

Positive Control	Methyl Methane Sulfonate	15	44	44	193	147	151*
Positive Control			39	41	183	155	151*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	62	106	119	64	60
			72	102	119	55
			53	99	105	66
			55	93	94	57
Test Chemical	2,3,5,6-Tetrachloro-4-nitroanisole	2	81	85	159	65	60
			71	62	118	55	60
		4	74	82	144	65	57
			83	92	124	50	57
		6	71	56	122	57	54
			86	45	132	51	54
		8	42	12	188	148	125*
			63	14	191	102	125*
		10

Positive Control	Methyl Methane Sulfonate	15	26	27	184	233	259*
Positive Control			18	18	151	285	259*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	88	102	47	18	23
			86	99	64	25
			78	95	38	16
			96	104	95	33
Test Chemical	2,3,5,6-Tetrachloro-4-nitroanisole	1	87	103	57	22
			152r	176	71	16
		3	78	78	81	34	37
			78	74	90	39	37
		5	97	52	184	63	73*
			81	57	201	83	73*
		7	56	21	217	130	118*
			65	18	206	106	118*
		9	29	4	200	230	174*
			48	9	171	118	174*
		11

Positive Control	Methyl Methane Sulfonate	15	43	26	63	48	59*
			45	28	94	69	59*
	Methyl Methane Sulfonate	250	51	64	214	141	159*
			46	50	245	178	159*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination