Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01174-0008-0000-3 |
Chemical Name: | 2,3,5,6-Tetrachloro-4-nitroanisole |
CASRN: | 2438-88-2 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | 665415 |
Study Result: | Positive |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Acetone | 0 | 64 | 97 | 89 | 47 | 52 |
69 | 101 | 98 | 47 | ||||
62 | 98 | 126 | 68 | ||||
53 | 104 | 76 | 48 | ||||
Test Chemical | 2,3,5,6-Tetrachloro-4-nitroanisole | 1.25 | 78 | 110 | 133 | 57 | 56 |
82 | 109 | 134 | 54 | ||||
2.5 | 67 | 89 | 120 | 60 | 64 | ||
69 | 95 | 142 | 69 | ||||
5 | 83 | 92 | 125 | 50 | 49 | ||
74 | 99 | 106 | 48 | ||||
10 | 64 | 32 | 127 | 66 | 65 | ||
47 | 25 | 90 | 64 | ||||
20 | |||||||
Positive Control | Methyl Methane Sulfonate | 15 | 44 | 44 | 193 | 147 | 151* |
39 | 41 | 183 | 155 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Acetone | 0 | 62 | 106 | 119 | 64 | 60 |
72 | 102 | 119 | 55 | ||||
53 | 99 | 105 | 66 | ||||
55 | 93 | 94 | 57 | ||||
Test Chemical | 2,3,5,6-Tetrachloro-4-nitroanisole | 2 | 81 | 85 | 159 | 65 | 60 |
71 | 62 | 118 | 55 | ||||
4 | 74 | 82 | 144 | 65 | 57 | ||
83 | 92 | 124 | 50 | ||||
6 | 71 | 56 | 122 | 57 | 54 | ||
86 | 45 | 132 | 51 | ||||
8 | 42 | 12 | 188 | 148 | 125* | ||
63 | 14 | 191 | 102 | ||||
10 | |||||||
Positive Control | Methyl Methane Sulfonate | 15 | 26 | 27 | 184 | 233 | 259* |
18 | 18 | 151 | 285 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Acetone | 0 | 88 | 102 | 47 | 18 | 23 |
86 | 99 | 64 | 25 | ||||
78 | 95 | 38 | 16 | ||||
96 | 104 | 95 | 33 | ||||
Test Chemical | 2,3,5,6-Tetrachloro-4-nitroanisole | 1 | 87 | 103 | 57 | 22 | |
152r | 176 | 71 | 16 | ||||
3 | 78 | 78 | 81 | 34 | 37 | ||
78 | 74 | 90 | 39 | ||||
5 | 97 | 52 | 184 | 63 | 73* | ||
81 | 57 | 201 | 83 | ||||
7 | 56 | 21 | 217 | 130 | 118* | ||
65 | 18 | 206 | 106 | ||||
9 | 29 | 4 | 200 | 230 | 174* | ||
48 | 9 | 171 | 118 | ||||
11 | |||||||
Positive Control | Methyl Methane Sulfonate | 15 | 43 | 26 | 63 | 48 | 59* |
45 | 28 | 94 | 69 | ||||
Methyl Methane Sulfonate | 250 | 51 | 64 | 214 | 141 | 159* | |
46 | 50 | 245 | 178 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination