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Study Information
Study Attribute Value
CEBS Accession Number: 002-01188-0001-0000-1
Chemical Name: 2,3-Dichloronitrobenzene
CASRN: 3209-22-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 913666_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Test Failure 
No Activation  2  Weakly Positive 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Test Failure 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 07/24/1984    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 479 0.457 9.58 26.0 0.000
Test Chemical 2,3-Dichloronitrobenzene 
16.7       
 50 1041 455 0.437 9.10 26.0 -4.280 
50         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.01      
 5 105 217 2.067 43.40 26.0 352.596
Trend: -0.668        
Probability: 0.748        

Trial #: 2    Activation: No Activation    Date: 08/01/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1043 519 0.498 10.38 26.0 0.000
Test Chemical 2,3-Dichloronitrobenzene 
10.2       
 50 1047 540 0.516 10.80 26.0 3.649 
25.4       
 50 1040 614 0.590 12.28 34.5 18.646 
50.8       
 50 1037 682 0.658 13.64 34.5 32.167 
75.8       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1040 612 0.588 12.24 26.0 18.259
Mitomycin-C 
 0.01      
 5 105 239 2.276 47.80 26.0 357.431
Trend: 5.357        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 08/14/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 416 0.397 8.32 26.0 0.000
Test Chemical 2,3-Dichloronitrobenzene 
30.2       
 50 1045 521 0.499 10.42 33.0 25.600 
40.2       
 50 1044 481 0.461 9.62 33.0 16.068 
50.2       
 50 1040 513 0.493 10.26 33.0 24.266 
60.5       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1049 661 0.630 13.22 26.0 58.743
Mitomycin-C 
 0.01      
 5 105 210 2.000 42.00 26.0 403.846
Trend: 2.578        
Probability: 0.005        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/24/1984    Trial Call: Test Failure   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 456 0.435 9.12 26.0 0.000
Test Chemical 2,3-Dichloronitrobenzene 
50         
 50 1049 437 0.417 8.74 26.0 -4.258 
166.7       
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
20         
 5 105 210 2.000 42.00 26.0 359.649
Trend: -0.650        
Probability: 0.742        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 08/01/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1043 486 0.466 9.72 26.0 0.000
Test Chemical 2,3-Dichloronitrobenzene 
75.8       
 50 1040 602 0.579 12.04 26.0 24.226 
101         
 50 1042 601 0.577 12.02 26.0 23.781 
150.8       
 50 1040 785 0.755 15.70 34.5 61.989 
201         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1039 807 0.777 16.14 26.0 66.689
Cyclophosphamide 
2         
 5 104 238 2.288 47.60 26.0 391.125
Trend: 8.269        
Probability: 0.000