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Study Information
Study Attribute Value
CEBS Accession Number: 002-01188-0002-0000-2
Chemical Name: 2,3-Dichloronitrobenzene
CASRN: 3209-22-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 913666_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Questionable 

Trial #:1   Activation: No Activation    Date: 08/23/1984    Harvest Time: 20.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 4 0.040 3.0 1 0.010 1.0 3 0.030 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 2,3-Dichloronitrobenzene 
125.8       
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
150         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
200         
 100 4 0.040 3.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0 
250         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.04      
 100 31 0.310 26.0 15 0.150 14.0 15 0.150 13.0 1 0.010 1.0
Mitomycin-C 
 0.06      
 25 15 0.600 56.0 8 0.320 32.0 7 0.280 24.0 0 0.000 0.0
Trend: 0.406 0.082 0.933  
Probability: 0.342 0.467 0.175  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 09/19/1984    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 50 1 0.020 2.0 0 0.000 0.0 1 0.020 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 50 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,3-Dichloronitrobenzene 
100         
 50 3 0.060 6.0 2 0.040 4.0 1 0.020 2.0 0 0.000 0.0 
119.3       
 50 33 0.660 40.0 22 0.440 32.0 11 0.220 20.0 0 0.000 0.0 
140.9       
 20 21 1.050 55.0 15 0.750 40.0 6 0.300 30.0 0 0.000 0.0 
150.9       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
6.25      
 50 4 0.080 8.0 2 0.040 4.0 2 0.040 4.0 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 19 0.760 36.0 12 0.480 36.0 6 0.240 20.0 1 0.040 4.0
Trend: 6.605 5.591 4.676  
Probability: 0.000 0.000 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 08/23/1984    Harvest Time: 20.5 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 2,3-Dichloronitrobenzene 
160         
 100 9 0.090 7.0 6 0.060 4.0 3 0.030 3.0 0 0.000 0.0 
170         
 100 11 0.110 9.0 8 0.080 7.0 3 0.030 2.0 0 0.000 0.0 
200         
 100 9 0.090 6.0 3 0.030 3.0 6 0.060 4.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
6.25      
 100 16 0.160 14.0 10 0.100 9.0 6 0.060 6.0 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 26 1.040 52.0 14 0.560 40.0 12 0.480 32.0 0 0.000 0.0
Trend: 1.094 0.732 1.249  
Probability: 0.137 0.232 0.106