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Study Information
Study Attribute Value
CEBS Accession Number: 002-01204-0002-0000-1
Chemical Name: 2,4,5-Trimethoxybenzaldehyde
CASRN: 4460-86-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 882349_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 
No Activation  3  Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #:1   Activation: No Activation    Date: 12/20/1988    Harvest Time: 22 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: 2,4,5-Trimethoxybenzaldehyde 
1000         
 200 21 0.105 10.5 8 0.040 4.0 13 0.065 6.5 0 0.000 0.0 
1500         
 200 19 0.095 9.0 7 0.035 3.5 12 0.060 5.5 0 0.000 0.0 
2000         
 200 68 0.340 24.5 19 0.095 8.5 47 0.235 16.5 2 0.010 2.0
Positive Control: Mitomycin-C 
 0.125     
 200 245 1.225 62.0 121 0.605 42.5 94 0.470 37.0 30 0.150 3.0
Mitomycin-C 
 0.25      
 25 73 2.920 96.0 29 1.160 72.0 34 1.360 80.0 10 0.400 1.0
Trend: 6.887 3.932 5.554  
Probability: 0.000 0.000 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 12/15/1988    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Test Chemical: 2,4,5-Trimethoxybenzaldehyde 
1000         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0 
1500         
 200 5 0.025 2.0 0 0.000 0.0 5 0.025 2.0 0 0.000 0.0 
2000         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
5         
 200 29 0.145 14.0 9 0.045 4.5 20 0.100 10.0 0 0.000 0.0
Cyclophosphamide 
7.5       
 50 18 0.360 32.0 3 0.060 6.0 14 0.280 28.0 1 0.020 1.0
Trend: -0.354 0.000 -0.354  
Probability: 0.638 0.000 0.638  


Trial #:2   Activation: No Activation    Date: 08/23/1984    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 2,4,5-Trimethoxybenzaldehyde 
25         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0 
50         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
100         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
250         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 12 0.120 9.0 7 0.070 6.0 5 0.050 3.0 0 0.000 0.0
Mitomycin-C 
1         
 50 36 0.720 50.0 30 0.600 46.0 6 0.120 12.0 0 0.000 0.0
Trend: -0.038 -0.355 0.340  
Probability: 0.515 0.639 0.367  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 04/23/1985    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,4,5-Trimethoxybenzaldehyde 
750         
 100 2 0.020 1.0 0 0.000 0.0 2 0.020 1.0 0 0.000 0.0 
1000         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
1500         
 100 6 0.060 6.0 4 0.040 4.0 2 0.020 2.0 0 0.000 0.0 
2000         
 100 6 0.060 5.0 2 0.020 2.0 4 0.040 3.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
10         
 100 40 0.400 30.0 25 0.250 21.0 15 0.150 14.0 0 0.000 0.0
Cyclophosphamide 
15         
 50 42 0.840 56.0 25 0.500 42.0 17 0.340 24.0 0 0.000 0.0
Trend: 2.036 2.430 1.741  
Probability: 0.021 0.008 0.041  


Trial #:3   Activation: No Activation    Date: 08/23/1984    Harvest Time: 22 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 2,4,5-Trimethoxybenzaldehyde 
750         
 100 6 0.060 6.0 3 0.030 3.0 3 0.030 3.0 0 0.000 0.0 
1000         
 100 6 0.060 6.0 4 0.040 4.0 2 0.020 2.0 0 0.000 0.0 
1500         
 100 10 0.100 8.0 4 0.040 4.0 6 0.060 5.0 0 0.000 0.0 
2000         
 100 28 0.280 18.0 9 0.090 8.0 18 0.180 12.0 1 0.010 1.0
Positive Control: Mitomycin-C 
 0.125     
 100 12 0.120 9.0 7 0.070 6.0 5 0.050 3.0 0 0.000 0.0
Mitomycin-C 
1         
 50 36 0.720 50.0 30 0.600 46.0 6 0.120 12.0 0 0.000 0.0
Trend: 4.217 2.775 3.629  
Probability: 0.000 0.003 0.000  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 08/23/1984    Harvest Time: 13 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,4,5-Trimethoxybenzaldehyde 
750         
 100 5 0.050 2.0 0 0.000 0.0 5 0.050 2.0 0 0.000 0.0 
1000         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
1500         
 100 10 0.100 10.0 3 0.030 3.0 7 0.070 7.0 0 0.000 0.0 
2000         
 100 27 0.270 25.0 4 0.040 3.0 23 0.230 22.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 59 0.590 33.0 33 0.330 21.0 26 0.260 19.0 0 0.000 0.0
Trend: 6.957 2.454 6.449  
Probability: 0.000 0.007 0.000