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Study Information
Study Attribute Value
CEBS Accession Number: 002-01204-0005-0000-4
Chemical Name: 2,4,5-Trimethoxybenzaldehyde
CASRN: 4460-86-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 041214
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 100 69 27 39
    78 102 76 32
    63 112 103 55
    57 86 71 41
Test Chemical 2,4,5-Trimethoxybenzaldehyde 
62.5       
 99 81 105 35 44
    65 81 105 54
  
125         
 77 47 79 34 37
    52 49 61 39
  
250         
 106 53 107 34 31
    83 57 73 29
  
500         
 75 47 90 40 40
    86 56 102 39
  
1000         
 88 66 84 32 30
    85 67 71 28
Positive Control Methyl Methane Sulfonate 
15         
 14 13 136 324 273*
    20 16 132 222

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 44r 77 72 55 32
    45r 69 85.5 63
    59 103 60 34
    69 97 63 31
Test Chemical 2,4,5-Trimethoxybenzaldehyde 
700         
 56# 68 52.5 31
  
900         
 39 59 100 85 69*
    55# 49 88.5 53
  
1100         
 33 33 111 112 99*
    59# 63 152 86
  
1300         
 38# 20 145.5 129 110*
    44 38 121 91
  
1700         
   
Positive Control Methyl Methane Sulfonate 
15         
 36 32 93 85 105*
    29 29 107 124

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87 102 143 55 60
    82 113 140 57
    79 95 167 71
    107 90 187 59
Test Chemical 2,4,5-Trimethoxybenzaldehyde 
1100         
 72 51 157 73 76
    69 51 163 79
  
1200         
 81 43 199 82 92
    92 31 281 102
  
1300         
 92 43 305 111 107*
    103 33 315 102
  
1400         
 57 14 289 170 168*
    53 24 264 166
  
1500         
  
1600         
   
Positive Control Methyl Methane Sulfonate 
15         
 35 26 271 257 220*
    58 30 319 183

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination