Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01204-0005-0000-4
Chemical Name:	2,4,5-Trimethoxybenzaldehyde
CASRN:	4460-86-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	041214
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	100	69	27	39
			78	102	76	32
			63	112	103	55
			57	86	71	41
Test Chemical	2,4,5-Trimethoxybenzaldehyde	62.5	99	81	105	35	44
			65	81	105	54	44
		125	77	47	79	34	37
			52	49	61	39	37
		250	106	53	107	34	31
			83	57	73	29	31
		500	75	47	90	40	40
			86	56	102	39	40
		1000	88	66	84	32	30
			85	67	71	28	30
Positive Control	Methyl Methane Sulfonate	15	14	13	136	324	273*
Positive Control			20	16	132	222	273*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	44r	77	72	55	32
			45r	69	85.5	63
			59	103	60	34
			69	97	63	31
Test Chemical	2,4,5-Trimethoxybenzaldehyde	700	56#	68	52.5	31
		900	39	59	100	85	69*
			55#	49	88.5	53	69*
		1100	33	33	111	112	99*
			59#	63	152	86	99*
		1300	38#	20	145.5	129	110*
			44	38	121	91	110*
		1700

Positive Control	Methyl Methane Sulfonate	15	36	32	93	85	105*
Positive Control			29	29	107	124	105*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	102	143	55	60
			82	113	140	57
			79	95	167	71
			107	90	187	59
Test Chemical	2,4,5-Trimethoxybenzaldehyde	1100	72	51	157	73	76
			69	51	163	79	76
		1200	81	43	199	82	92
			92	31	281	102	92
		1300	92	43	305	111	107*
			103	33	315	102	107*
		1400	57	14	289	170	168*
			53	24	264	166	168*
		1500
		1600

Positive Control	Methyl Methane Sulfonate	15	35	26	271	257	220*
Positive Control			58	30	319	183	220*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination