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Study Information
Study Attribute Value
CEBS Accession Number: 002-01205-0002-0000-2
Chemical Name: 2,4,5-Trimethylaniline
CASRN: 137-17-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 005305_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
No Activation  3  Weakly Positive* 
No Activation  4  Positive 

Trial #:1   Activation: No Activation    Date: 12/03/1985    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,4,5-Trimethylaniline 
755         
 200 65 0.325 12.5 18 0.090 6.0 27 0.135 9.0 20 0.100 2.0 
865         
 200 24 0.120 7.0 11 0.055 5.0 13 0.065 3.5 0 0.000 0.0 
1080         
 200 86 0.430 22.0 20 0.100 7.5 46 0.230 15.5 20 0.100 2.0
Positive Control: Mitomycin-C 
 0.2       
 200 30 0.150 12.0 9 0.045 4.0 20 0.100 8.5 1 0.005 1.0
Mitomycin-C 
5         
 10 65 6.500 100.0 6 0.600 30.0 9 0.900 40.0 50 5.000 5.0
Trend: 6.573 3.275 5.397  
Probability: 0.000 0.001 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 10/17/1985    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,4,5-Trimethylaniline 
47.1       
 200 3 0.015 1.5 0 0.000 0.0 2 0.010 1.0 1 0.005 1.0 
142         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
471         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0 
940         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 50 24 0.480 36.0 11 0.220 20.0 12 0.240 18.0 1 0.020 1.0
Trend: 0.690 -1.840 1.905  
Probability: 0.245 0.967 0.028  


Trial #:2   Activation: No Activation    Date: 10/17/1985    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0
Test Chemical: 2,4,5-Trimethylaniline 
47.1       
 200 8 0.040 4.0 0 0.000 0.0 8 0.040 4.0 0 0.000 0.0 
142         
 200 8 0.040 3.5 0 0.000 0.0 8 0.040 3.5 0 0.000 0.0 
471         
 200 13 0.065 6.0 1 0.005 0.5 12 0.060 5.5 0 0.000 0.0 
940         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
1         
 200 82 0.410 27.0 22 0.110 10.0 50 0.250 20.5 10 0.050 1.0
Mitomycin-C 
5         
 10 14 1.400 80.0 8 0.800 60.0 5 0.500 40.0 1 0.100 1.0
Trend: 1.889 1.382 1.662  
Probability: 0.029 0.084 0.048  


Trial #:3   Activation: No Activation    Date: 11/21/1985    Harvest Time: 21 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0
Test Chemical: 2,4,5-Trimethylaniline 
104         
 200 7 0.035 3.5 2 0.010 1.0 5 0.025 2.5 0 0.000 0.0 
207         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0 
620         
 72 48 0.667 26.4 4 0.056 4.2 24 0.333 20.8 20 0.278 2.0 
830         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.2       
 200 31 0.155 12.5 5 0.025 2.5 25 0.125 10.0 1 0.005 1.0
Mitomycin-C 
5         
 10 84 8.400 100.0 2 0.200 10.0 2 0.200 20.0 80 8.000 8.0
Trend: 6.590 1.899 6.041  
Probability: 0.000 0.029 0.000  


Trial #:4   Activation: No Activation    Date: 11/21/1985    Harvest Time: 21 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0
Test Chemical: 2,4,5-Trimethylaniline 
415         
 200 103 0.515 6.5 2 0.010 1.0 1 0.005 0.5 100 0.500 10.0 
620         
 200 61 0.305 6.0 7 0.035 2.0 4 0.020 1.5 50 0.250 5.0 
830         
 47 110 2.340 57.4 5 0.106 8.5 25 0.532 38.3 80 1.702 8.0 
1040         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.2       
 200 31 0.155 12.5 5 0.025 2.5 25 0.125 10.0 1 0.005 1.0
Mitomycin-C 
5         
 10 84 8.400 100.0 2 0.200 10.0 2 0.200 20.0 80 8.000 8.0
Trend: 7.837 2.963 7.082  
Probability: 0.000 0.002 0.000