Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01209-0009-0000-3
Chemical Name:	2,4,6-Trichlorophenol
CASRN:	88-06-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	736391
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	78	92	60	26	31
			74	92	106	48
			89	101	55	21
			82	115	70	28
Test Chemical	2,4,6-Trichlorophenol	6.25	97	89	130	45	36
			86	94	68	27	36
		12.5	67	78	70	35	27
			69	81	41	20	27
		25	66	79	94	48	42
			83#	73	90	36	42
		50	78	66	98	42	42
			73	70	91	42	42
		100	48	11	122	85	75*
			60	10	119	66	75*
		200

Positive Control	Methyl Methane Sulfonate	15	48	40	163	114	102*
			43	40	116	91	102*
	Methyl Methane Sulfonate	250	63	99	280	149	142*
			64	75	255	134	142*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	97	96	129	45	41
			114	90	141	41
			92	100	106	38
			109	115	126	39
Test Chemical	2,4,6-Trichlorophenol	10	92	106	141	51	51
			104	89	159	51	51
		20	76	88	114	50	58
			80	87	155	65	58
		40	84	68	174	69	60
			99	74	154	52	60
		80	79	26	199	85	81*
			91	19	212	78	81*
		120	93	11	385	138	185*
			71	9	496	233	185*
		160

Positive Control	Methyl Methane Sulfonate	15	38	25	207	183	173*
			45	31	219	162	173*
	Methyl Methane Sulfonate	250	64	70	455	236	237*
			56	71	403	238	237*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination