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Study Information
Study Attribute Value
CEBS Accession Number: 002-01215-0008-0000-9
Chemical Name: 2,4-Diaminoanisole sulfate
CASRN: 39156-41-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G45600
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
110 114 67 20 23
    109 86 82 25
Test Chemical 2,4-Diaminoanisole sulfate
10.3       
87 85 124 47 47*
    83 84 118 47
 
14.7       
83 76 131 53 48*
    82 64 108 44
 
21         
78 53 104 44 44*
    103 65 133 43
 
30         
81 50 143 59 56*
    92 55 148 53
 
42.9       
88 35 245 93 86*
    111 43 262 79
 
61.3       
69 18 288 139 137*
    70 13 282 135
 
87.5       
60 9 281 156 174*
    47 8 273 192
Positive Control Ethyl Methane Sulfonate
500         
43 37 826 645 616*
    56 51 987 588

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
88 98 70 27 28
    101 102 86 28
Test Chemical 2,4-Diaminoanisole sulfate
21         
92 102 73 27 30
    81 95 83 34
 
30         
91 88 91 33 33
    110 119 111 34
 
42.9       
81 69 113 47 42
    99 74 110 37
 
61.3       
102 63 121 39 38
    82 49 91 37
 
87.5       
83 37 125 50 54*
    95 38 167 58
 
125         
70 20 145 69 80*
    58 16 156 90
Positive Control Ethyl Methane Sulfonate
500         
60 47 800 443 470*
    54 42 801 498

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
63 92 231 122 109
    85 108 247 97
Test Chemical 2,4-Diaminoanisole sulfate
3.01      
71 59 388 183 208*
    58 47 410 234
 
4.3       
57 39 388 227 242*
    57 37 436 257
 
6.14      
49 25 404 274 300*
    44 27 426 325
 
7.68      
48 19 383 264 249*
    52 28 367 233
 
9.6       
54 20 456 281 286*
    52 23 449 291
 
12         
55 24 499 301 296*
    54 18 472 292
 
15         
52 14 476 307 288*
    55 16 445 270
Positive Control 3-Methylcholanthrene
5         
44 21 549 414 412*
    36 19 446 409

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
75 105 213 94 92
    83 95 224 90
Test Chemical 2,4-Diaminoanisole sulfate
2.11      
47 23 346 245 203*
    55 33 265 162
 
3.01      
85 35 291 115 130
    64 26 279 145
 
4.3       
50 22 306 206 205*
    49 18 299 205
 
6.14      
39 9 305 260 237*
    46 10 296 215
 
7.68      
36 6 303 283 296*
    33 5 308 308
 
9.6       
42 9 300 240 230*
    44 11 288 219
 
12         
31 5 287 314 303*
    30 4 263 292
Positive Control 3-Methylcholanthrene
5         
20 8 267 441 503*
    18 6 311 565

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination