Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01215-0008-0000-9
Chemical Name:	2,4-Diaminoanisole sulfate
CASRN:	39156-41-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G45600
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	110	114	67	20	23
Vehicle Control			109	86	82	25	23
Test Chemical	2,4-Diaminoanisole sulfate	10.3	87	85	124	47	47*
			83	84	118	47	47*
		14.7	83	76	131	53	48*
			82	64	108	44	48*
		21	78	53	104	44	44*
			103	65	133	43	44*
		30	81	50	143	59	56*
			92	55	148	53	56*
		42.9	88	35	245	93	86*
			111	43	262	79	86*
		61.3	69	18	288	139	137*
			70	13	282	135	137*
		87.5	60	9	281	156	174*
			47	8	273	192	174*
Positive Control	Ethyl Methane Sulfonate	500	43	37	826	645	616*
Positive Control			56	51	987	588	616*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	98	70	27	28
Vehicle Control			101	102	86	28	28
Test Chemical	2,4-Diaminoanisole sulfate	21	92	102	73	27	30
			81	95	83	34	30
		30	91	88	91	33	33
			110	119	111	34	33
		42.9	81	69	113	47	42
			99	74	110	37	42
		61.3	102	63	121	39	38
			82	49	91	37	38
		87.5	83	37	125	50	54*
			95	38	167	58	54*
		125	70	20	145	69	80*
			58	16	156	90	80*
Positive Control	Ethyl Methane Sulfonate	500	60	47	800	443	470*
Positive Control			54	42	801	498	470*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	63	92	231	122	109
Vehicle Control			85	108	247	97	109
Test Chemical	2,4-Diaminoanisole sulfate	3.01	71	59	388	183	208*
			58	47	410	234	208*
		4.3	57	39	388	227	242*
			57	37	436	257	242*
		6.14	49	25	404	274	300*
			44	27	426	325	300*
		7.68	48	19	383	264	249*
			52	28	367	233	249*
		9.6	54	20	456	281	286*
			52	23	449	291	286*
		12	55	24	499	301	296*
			54	18	472	292	296*
		15	52	14	476	307	288*
			55	16	445	270	288*
Positive Control	3-Methylcholanthrene	5	44	21	549	414	412*
Positive Control			36	19	446	409	412*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75	105	213	94	92
Vehicle Control			83	95	224	90	92
Test Chemical	2,4-Diaminoanisole sulfate	2.11	47	23	346	245	203*
			55	33	265	162	203*
		3.01	85	35	291	115	130
			64	26	279	145	130
		4.3	50	22	306	206	205*
			49	18	299	205	205*
		6.14	39	9	305	260	237*
			46	10	296	215	237*
		7.68	36	6	303	283	296*
			33	5	308	308	296*
		9.6	42	9	300	240	230*
			44	11	288	219	230*
		12	31	5	287	314	303*
			30	4	263	292	303*
Positive Control	3-Methylcholanthrene	5	20	8	267	441	503*
Positive Control			18	6	311	565	503*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination