Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01215-0009-0000-0
Chemical Name:	2,4-Diaminoanisole sulfate
CASRN:	39156-41-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G63131
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	96	92	129	45	48
Vehicle Control			87	93	136	52	48
Test Chemical	2,4-Diaminoanisole sulfate	3.93	73	78	116	53	46
			84	108	97	38	46
		7.87	90	94	133	49	49
			72	92	105	49	49
		15.7	73	71	128	58	55
			82	68	127	52	55
		31.5	97	58	216	75	86*
			77	58	223	97	86*
		63	82	18	467	190	238*
			60	17	516	287	238*
		126

Positive Control	Ethyl Methane Sulfonate	500	39	28	814	696	664*
Positive Control			43	27	809	632	664*
Negative Control	Negative (Not Specified)	0	98	99	101	34	34*
Negative Control			97	115	96	33	34*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	92	93	64	23	21
Vehicle Control			96	108	52	18	21
Test Chemical	2,4-Diaminoanisole sulfate	20	78#	69	64	27	29
			69#	69	63	31	29
		40	94	52	124	44	46*
			89	61	130	49	46*
		60	85	27	246	97	103*
			83	27	273	109	103*
		100	65	5	514	264	232*
			52	3	312	201	232*
Positive Control	Ethyl Methane Sulfonate	500	82	64	542	221	282*
Positive Control			68	47	701	344	282*
Negative Control	Negative (Not Specified)	0	97	95	50	17	23*
Negative Control			82	105	69	28	23*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	99	79	67	23	17
			103	118	48	16
			94	108	40	14
			103	95	45	15
Test Chemical	2,4-Diaminoanisole sulfate	12.5	96	77	122	43	41*
			83	82	98	40	41*
		25	96	52	181	63	83*
			72	39	223	104	83*
		50	64	9	285	150	153*
			66	15	307	156	153*
		75	32	3	430	446	427*
			27	2	386	482
			31	2	331	354
		100


Positive Control	Ethyl Methane Sulfonate	500	32	13	751	778	734*
			36	23	785	737
			40	22	822	688

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	91	105	111	41
Test Chemical	2,4-Diaminoanisole sulfate	0.975	88#	100	88.5	34	37
			103	103	123	40	37
		1.95	100	108	165	55	51
			96	117	136	47	51
		3.9	83	80	182	73	70
			82	91	163	66	70
		7.8	90	61	206	77	76*
			98	76	223	76	76*
		15.6	96	66	235	81
Positive Control	3-Methylcholanthrene	5	70	34	765	364	357*
Positive Control			64	37	675	350	357*
Negative Control	Negative (Not Specified)	0	78#	98	96	41	43*
Negative Control			96	97	131	45	43*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	64	87	127	66	69
			82	124	143	58
			98	114	186	63
			77	75	210	91
Test Chemical	2,4-Diaminoanisole sulfate	10	32	18	169	176	146*
			61	35	212	116	146*
		15	63	35	218	115	135*
			64	33	299	155	135*
		20	46	21	151	109	125*
			37	21	158	141	125*
		25	51	26	256	168	170*
			53	21	276	172	170*
		30	42	19	221	174	174*
			35	20	182	174	174*
		40	35	17	171	164	154*
			58	28	252	144	154*
Positive Control	3-Methylcholanthrene	5	41	14	521	424	433*
Positive Control			48	15	635	443	433*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	83	105	89	36	32
			87	96	78	30
			85	109	75	30
			87	90	86	33
Test Chemical	2,4-Diaminoanisole sulfate	12.5	100	88	219	73	77*
			81	89	198	81	77*
		25	79	92	202	85	81*
			76	66	176	77	81*
		50	76	73	137	60	64*
			64	42	131	69	64*
		75	82	32	238	97	136*
			63	26	331	175	136*
		100	75	19	242	108	102*
			86#	29	247	96	102*
		150

Positive Control	3-Methylcholanthrene	5	39#	10	366	317	504*
			22	4	337	518
			23	4	464	677

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination