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Study Information
Study Attribute Value
CEBS Accession Number: 002-01215-0009-0000-0
Chemical Name: 2,4-Diaminoanisole sulfate
CASRN: 39156-41-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G63131
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 96 92 129 45 48
    87 93 136 52
Test Chemical 2,4-Diaminoanisole sulfate 
3.93      
 73 78 116 53 46
    84 108 97 38
  
7.87      
 90 94 133 49 49
    72 92 105 49
  
15.7       
 73 71 128 58 55
    82 68 127 52
  
31.5       
 97 58 216 75 86*
    77 58 223 97
  
63         
 82 18 467 190 238*
    60 17 516 287
  
126         
   
Positive Control Ethyl Methane Sulfonate 
500         
 39 28 814 696 664*
    43 27 809 632
Negative Control Negative (Not Specified) 
 0         
 98 99 101 34 34*
    97 115 96 33

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 92 93 64 23 21
    96 108 52 18
Test Chemical 2,4-Diaminoanisole sulfate 
20         
 78# 69 64 27 29
    69# 69 63 31
  
40         
 94 52 124 44 46*
    89 61 130 49
  
60         
 85 27 246 97 103*
    83 27 273 109
  
100         
 65 5 514 264 232*
    52 3 312 201
Positive Control Ethyl Methane Sulfonate 
500         
 82 64 542 221 282*
    68 47 701 344
Negative Control Negative (Not Specified) 
 0         
 97 95 50 17 23*
    82 105 69 28

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 99 79 67 23 17
    103 118 48 16
    94 108 40 14
    103 95 45 15
Test Chemical 2,4-Diaminoanisole sulfate 
12.5       
 96 77 122 43 41*
    83 82 98 40
  
25         
 96 52 181 63 83*
    72 39 223 104
  
50         
 64 9 285 150 153*
    66 15 307 156
  
75         
 32 3 430 446 427*
    27 2 386 482
    31 2 331 354
  
100         
   
   
Positive Control Ethyl Methane Sulfonate 
500         
 32 13 751 778 734*
    36 23 785 737
    40 22 822 688

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 91 105 111 41
Test Chemical 2,4-Diaminoanisole sulfate 
 0.975     
 88# 100 88.5 34 37
    103 103 123 40
  
1.95      
 100 108 165 55 51
    96 117 136 47
  
3.9       
 83 80 182 73 70
    82 91 163 66
  
7.8       
 90 61 206 77 76*
    98 76 223 76
  
15.6       
 96 66 235 81
Positive Control 3-Methylcholanthrene 
5         
 70 34 765 364 357*
    64 37 675 350
Negative Control Negative (Not Specified) 
 0         
 78# 98 96 41 43*
    96 97 131 45

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 64 87 127 66 69
    82 124 143 58
    98 114 186 63
    77 75 210 91
Test Chemical 2,4-Diaminoanisole sulfate 
10         
 32 18 169 176 146*
    61 35 212 116
  
15         
 63 35 218 115 135*
    64 33 299 155
  
20         
 46 21 151 109 125*
    37 21 158 141
  
25         
 51 26 256 168 170*
    53 21 276 172
  
30         
 42 19 221 174 174*
    35 20 182 174
  
40         
 35 17 171 164 154*
    58 28 252 144
Positive Control 3-Methylcholanthrene 
5         
 41 14 521 424 433*
    48 15 635 443

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 83 105 89 36 32
    87 96 78 30
    85 109 75 30
    87 90 86 33
Test Chemical 2,4-Diaminoanisole sulfate 
12.5       
 100 88 219 73 77*
    81 89 198 81
  
25         
 79 92 202 85 81*
    76 66 176 77
  
50         
 76 73 137 60 64*
    64 42 131 69
  
75         
 82 32 238 97 136*
    63 26 331 175
  
100         
 75 19 242 108 102*
    86# 29 247 96
  
150         
   
Positive Control 3-Methylcholanthrene 
5         
 39# 10 366 317 504*
    22 4 337 518
    23 4 464 677

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination