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Study Information
Study Attribute Value
CEBS Accession Number: 002-01216-0009-0000-1
Chemical Name: 2,4-Diaminophenol dihydrochloride
CASRN: 137-09-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 299952
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 69 77 140 67 64
    48r 49 117 81
    80 140 129 54
    66 84 144 73
Test Chemical 2,4-Diaminophenol dihydrochloride 
 0.25      
 56# 64 95 56 53
    73 89 107 49
  
 0.5       
 69 86 158 76 72
    76 61 153 68
  
1         
 72 78 122 57 62
    63# 64 124.5 66
  
2         
 70 50 176 84 76
    77 52 157 68
  
4         
   
Positive Control Ethyl Methane Sulfonate 
250         
 45# 36 499.5 373 348*
    53 41 513 324

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 82 91 72 29 31
    117 139 109 31
    87 64 77 30
    87 105 85 33
Test Chemical 2,4-Diaminophenol dihydrochloride 
 0.25      
 73 86 100 46 42
    79 96 89 38
  
 0.5       
 80 85 101 42 38
    102 99 106 35
  
1         
 91 96 121 44 40
    80 101 84 35
  
2         
 87 56 95 36 42
    75 49 105 47
  
4         
 65 6 293 151 152*
    39 4 180 153
  
8         
   
Positive Control Ethyl Methane Sulfonate 
250         
 66 49 553 280 304*
    59 53 582 328

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 88 116 137 52 59
    97 110 185 64
    88 98 154 58
    83 76 150 60
Test Chemical 2,4-Diaminophenol dihydrochloride 
 0.5       
 86 98 174 68 67
    83 93 149 60
    64 92 141 73
  
1         
 75 75 157 70 77
    76 70 166 73
    68 91 179 88
  
2         
 70 45 224 107 109*
    66 56 221 112
    83 53 271 109
  
3         
 42 7 542 427 473*
    39 7 628 537
    47 8 644 457
  
4         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 79 67 606 257 285*
    62 61 581 313

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination