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Study Information
Study Attribute Value
CEBS Accession Number: 002-01217-0001-0000-4
Chemical Name: 2,4-Diaminotoluene (2,4-toluene diamine)
CASRN: 95-80-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 314882_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 10/29/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1040 467 0.449 9.34 27.0 0.000
Test Chemical 2,4-Diaminotoluene (2,4-toluene diamine) 
4.68      
 50 1046 532 0.509 10.64 27.0 13.265 
14.1       
 50 1037 605 0.583 12.10 27.0 29.925 
46.8       
 50 1035 970 0.937 19.40 31.0 108.712 
141         
 0 0 0 0.000 0.00 31.0 N/A
Positive Control Mitomycin-C 
 0.002     
 50 1047 590 0.564 11.80 27.0 25.494
Mitomycin-C 
 0.01      
 10 208 194 0.933 19.40 27.0 107.709
Trend: 14.188        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 12/03/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1043 481 0.461 9.62 26.5 0.000
Test Chemical 2,4-Diaminotoluene (2,4-toluene diamine) 
64.5       
 50 1040 777 0.747 15.54 26.5 62.004 
97         
 50 1037 1004 0.968 20.08 30.5 109.940 
130         
 50 1048 1288 1.229 25.76 30.5 166.498 
194         
 0 0 0 0.000 0.00 30.5 N/A
Positive Control Mitomycin-C 
 0.002     
 50 1049 614 0.585 12.28 26.5 26.921
Mitomycin-C 
 0.01      
 10 210 258 1.229 25.80 26.5 166.403
Trend: 19.718        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/29/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 455 0.434 9.10 26.0 0.000
Test Chemical 2,4-Diaminotoluene (2,4-toluene diamine) 
468         
 50 1039 728 0.701 14.56 26.0 61.386 
1410         
 50 1045 571 0.546 11.42 26.0 25.855 
4680         
 50 1046 614 0.587 12.28 26.0 35.203
Positive Control Cyclophosphamide 
 0.5       
 50 1052 770 0.732 15.40 26.0 68.587
Cyclophosphamide 
2.5       
 10 212 279 1.316 27.90 26.0 203.123
Trend: 2.967        
Probability: 0.002