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Study Information
Study Attribute Value
CEBS Accession Number: 002-01217-0002-0000-5
Chemical Name: 2,4-Diaminotoluene (2,4-toluene diamine)
CASRN: 95-80-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 314882_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Positive 
No Activation  3  Weakly Positive 

Trial #:1   Activation: No Activation    Date: 12/12/1985    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 9 0.045 4.0 3 0.015 1.5 6 0.030 3.0 0 0.000 0.0
Test Chemical: 2,4-Diaminotoluene (2,4-toluene diamine) 
98.5       
 100 116 1.160 25.0 6 0.060 5.0 7 0.070 7.0 103 1.030 13.0 
197         
 100 119 1.190 18.0 3 0.030 2.0 5 0.050 5.0 111 1.110 12.0 
492         
 72 100 1.389 41.7 5 0.069 6.9 26 0.361 13.9 69 0.958 15.0
Positive Control: Mitomycin-C 
 0.2       
 15 56 3.733 86.7 8 0.533 33.3 8 0.533 46.7 40 2.667 4.0
Mitomycin-C 
5         
 10 77 7.700 90.0 2 0.200 20.0 5 0.500 20.0 70 7.000 7.0
Trend: 6.988 1.857 2.956  
Probability: 0.000 0.032 0.002  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 11/14/1985    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 3 0.015 1.0 0 0.000 0.0 2 0.010 1.0 1 0.005 1.0
Test Chemical: 2,4-Diaminotoluene (2,4-toluene diamine) 
455         
 200 8 0.040 4.0 3 0.015 1.5 5 0.025 2.5 0 0.000 0.0 
1370         
 200 17 0.085 7.0 8 0.040 3.5 9 0.045 4.5 0 0.000 0.0 
4550         
 200 25 0.125 12.0 5 0.025 2.0 19 0.095 9.5 1 0.005 1.0
Positive Control: Cyclophosphamide 
50         
 25 46 1.840 68.0 9 0.360 24.0 17 0.680 48.0 20 0.800 2.0
Trend: 4.802 1.901 4.268  
Probability: 0.000 0.029 0.000  


Trial #:2   Activation: No Activation    Date: 12/12/1985    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 9 0.045 4.0 3 0.015 1.5 6 0.030 3.0 0 0.000 0.0
Test Chemical: 2,4-Diaminotoluene (2,4-toluene diamine) 
1480         
 100 144 1.440 16.0 2 0.020 1.0 2 0.020 1.0 140 1.400 14.0 
2950         
 200 119 0.595 16.0 7 0.035 2.5 14 0.070 6.5 98 0.490 17.0 
4920         
 100 164 1.640 19.0 1 0.010 1.0 2 0.020 2.0 161 1.610 17.0
Positive Control: Mitomycin-C 
 0.2       
 15 56 3.733 86.7 8 0.533 33.3 8 0.533 46.7 40 2.667 4.0
Mitomycin-C 
5         
 10 77 7.700 90.0 2 0.200 20.0 5 0.500 20.0 70 7.000 7.0
Trend: 4.129 0.256 0.861  
Probability: 0.000 0.399 0.195  


Trial #:3   Activation: No Activation    Date: 11/14/1985    Harvest Time: 10.5 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 6 0.030 2.5 1 0.005 0.5 5 0.025 2.0 0 0.000 0.0
Test Chemical: 2,4-Diaminotoluene (2,4-toluene diamine) 
13.7       
 200 9 0.045 4.0 2 0.010 1.0 7 0.035 3.0 0 0.000 0.0 
45.5       
 200 9 0.045 4.5 0 0.000 0.0 9 0.045 4.5 0 0.000 0.0 
137         
 200 36 0.180 9.0 1 0.005 0.5 24 0.120 8.0 11 0.055 2.0 
273         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
1         
 200 68 0.340 23.5 26 0.130 12.5 41 0.205 13.5 1 0.005 1.0
Mitomycin-C 
5         
 20 42 2.100 70.0 8 0.400 25.0 23 1.150 60.0 11 0.550 2.0
Trend: 2.901 -0.485 3.025  
Probability: 0.002 0.686 0.001