Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01217-0002-0000-5 |
Chemical Name: | 2,4-Diaminotoluene (2,4-toluene diamine) |
CASRN: | 95-80-7 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 314882_CA |
Study Result: | Positive |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Positive |
Induced Rat Liver S9 | 1_S9 | Positive |
No Activation | 2 | Positive |
No Activation | 3 | Weakly Positive |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 9 | 0.045 | 4.0 | 3 | 0.015 | 1.5 | 6 | 0.030 | 3.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,4-Diaminotoluene (2,4-toluene diamine) | 98.5 | 100 | 116 | 1.160 | 25.0 | 6 | 0.060 | 5.0 | 7 | 0.070 | 7.0 | 103 | 1.030 | 13.0 |
197 | 100 | 119 | 1.190 | 18.0 | 3 | 0.030 | 2.0 | 5 | 0.050 | 5.0 | 111 | 1.110 | 12.0 | ||
492 | 72 | 100 | 1.389 | 41.7 | 5 | 0.069 | 6.9 | 26 | 0.361 | 13.9 | 69 | 0.958 | 15.0 | ||
Positive Control: | Mitomycin-C | 0.2 | 15 | 56 | 3.733 | 86.7 | 8 | 0.533 | 33.3 | 8 | 0.533 | 46.7 | 40 | 2.667 | 4.0 |
Mitomycin-C | 5 | 10 | 77 | 7.700 | 90.0 | 2 | 0.200 | 20.0 | 5 | 0.500 | 20.0 | 70 | 7.000 | 7.0 | |
Trend: | 6.988 | 1.857 | 2.956 | ||||||||||||
Probability: | 0.000 | 0.032 | 0.002 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 3 | 0.015 | 1.0 | 0 | 0.000 | 0.0 | 2 | 0.010 | 1.0 | 1 | 0.005 | 1.0 |
Test Chemical: | 2,4-Diaminotoluene (2,4-toluene diamine) | 455 | 200 | 8 | 0.040 | 4.0 | 3 | 0.015 | 1.5 | 5 | 0.025 | 2.5 | 0 | 0.000 | 0.0 |
1370 | 200 | 17 | 0.085 | 7.0 | 8 | 0.040 | 3.5 | 9 | 0.045 | 4.5 | 0 | 0.000 | 0.0 | ||
4550 | 200 | 25 | 0.125 | 12.0 | 5 | 0.025 | 2.0 | 19 | 0.095 | 9.5 | 1 | 0.005 | 1.0 | ||
Positive Control: | Cyclophosphamide | 50 | 25 | 46 | 1.840 | 68.0 | 9 | 0.360 | 24.0 | 17 | 0.680 | 48.0 | 20 | 0.800 | 2.0 |
Trend: | 4.802 | 1.901 | 4.268 | ||||||||||||
Probability: | 0.000 | 0.029 | 0.000 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 9 | 0.045 | 4.0 | 3 | 0.015 | 1.5 | 6 | 0.030 | 3.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,4-Diaminotoluene (2,4-toluene diamine) | 1480 | 100 | 144 | 1.440 | 16.0 | 2 | 0.020 | 1.0 | 2 | 0.020 | 1.0 | 140 | 1.400 | 14.0 |
2950 | 200 | 119 | 0.595 | 16.0 | 7 | 0.035 | 2.5 | 14 | 0.070 | 6.5 | 98 | 0.490 | 17.0 | ||
4920 | 100 | 164 | 1.640 | 19.0 | 1 | 0.010 | 1.0 | 2 | 0.020 | 2.0 | 161 | 1.610 | 17.0 | ||
Positive Control: | Mitomycin-C | 0.2 | 15 | 56 | 3.733 | 86.7 | 8 | 0.533 | 33.3 | 8 | 0.533 | 46.7 | 40 | 2.667 | 4.0 |
Mitomycin-C | 5 | 10 | 77 | 7.700 | 90.0 | 2 | 0.200 | 20.0 | 5 | 0.500 | 20.0 | 70 | 7.000 | 7.0 | |
Trend: | 4.129 | 0.256 | 0.861 | ||||||||||||
Probability: | 0.000 | 0.399 | 0.195 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 200 | 6 | 0.030 | 2.5 | 1 | 0.005 | 0.5 | 5 | 0.025 | 2.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2,4-Diaminotoluene (2,4-toluene diamine) | 13.7 | 200 | 9 | 0.045 | 4.0 | 2 | 0.010 | 1.0 | 7 | 0.035 | 3.0 | 0 | 0.000 | 0.0 |
45.5 | 200 | 9 | 0.045 | 4.5 | 0 | 0.000 | 0.0 | 9 | 0.045 | 4.5 | 0 | 0.000 | 0.0 | ||
137 | 200 | 36 | 0.180 | 9.0 | 1 | 0.005 | 0.5 | 24 | 0.120 | 8.0 | 11 | 0.055 | 2.0 | ||
273 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 1 | 200 | 68 | 0.340 | 23.5 | 26 | 0.130 | 12.5 | 41 | 0.205 | 13.5 | 1 | 0.005 | 1.0 |
Mitomycin-C | 5 | 20 | 42 | 2.100 | 70.0 | 8 | 0.400 | 25.0 | 23 | 1.150 | 60.0 | 11 | 0.550 | 2.0 | |
Trend: | 2.901 | -0.485 | 3.025 | ||||||||||||
Probability: | 0.002 | 0.686 | 0.001 |