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Study Information
Study Attribute Value
CEBS Accession Number: 002-01226-0001-0000-4
Chemical Name: 2,4-Dimethoxyaniline hydrochloride
CASRN: 54150-69-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 434745_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Questionable 

Trial #: 1    Activation: No Activation    Date: 09/11/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 477 0.454 9.54 27.0 0.000
Test Chemical 2,4-Dimethoxyaniline hydrochloride 
16         
 50 1048 483 0.461 9.66 27.0 1.451 
50         
 50 1049 533 0.508 10.66 27.0 11.847 
160         
 50 1048 718 0.685 14.36 27.0 50.811 
500         
 0 0 0 0.000 0.00 27.0 N/A 
1600         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1048 614 0.586 12.28 27.0 28.967
Mitomycin-C 
 0.005     
 10 210 315 1.500 31.50 27.0 230.189
Trend: 7.388        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 10/16/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1051 440 0.419 8.80 26.5 0.000
Test Chemical 2,4-Dimethoxyaniline hydrochloride 
25         
 50 1049 498 0.475 9.96 26.5 13.398 
50         
 50 1050 498 0.474 9.96 26.5 13.290 
100         
 50 1048 656 0.626 13.12 31.5 49.518 
200         
 50 1052 1055 1.003 21.10 32.5 139.545 
300         
 50 1049 1191 1.135 23.82 32.5 171.198 
400         
 50 1049 1334 1.272 26.68 32.5 203.760 
500         
 0 0 0 0.000 0.00 32.5 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1049 639 0.609 12.78 26.5 45.504
Mitomycin-C 
 0.005     
 10 210 265 1.262 26.50 26.5 201.423
Trend: 30.383        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 09/11/1984    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 370 0.354 7.40 26.5 0.000
Test Chemical 2,4-Dimethoxyaniline hydrochloride 
50         
 50 1048 415 0.396 8.30 26.5 11.734 
160         
 50 1046 415 0.397 8.30 26.5 11.948 
500         
 50 1047 326 0.311 6.52 26.5 -12.144 
1600         
 50 1044 451 0.432 9.02 26.5 21.892 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1048 642 0.613 12.84 26.5 72.851
Cyclophosphamide 
 0.6       
 10 206 178 0.864 17.80 26.5 143.810
Trend: 1.178        
Probability: 0.119