COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-01226-0002-0000-5
Chemical Name: 2,4-Dimethoxyaniline hydrochloride
CASRN: 54150-69-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 434745_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Questionable 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #:1   Activation: No Activation    Date: 10/04/1984    Harvest Time: 13.5 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 2 0.020 1.0 2 0.020 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,4-Dimethoxyaniline hydrochloride 
300         
 100 9 0.090 7.0 5 0.050 4.0 4 0.040 4.0 0 0.000 0.0 
500         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
1000         
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0 
1600         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
3000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 50 0.500 33.0 36 0.360 26.0 14 0.140 14.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 20 0.400 32.0 14 0.280 26.0 6 0.120 10.0 0 0.000 0.0
Trend: -0.110 -0.646 0.248  
Probability: 0.544 0.741 0.402  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 10/04/1984    Harvest Time: 14.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,4-Dimethoxyaniline hydrochloride 
500         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
1000         
 100 10 0.100 9.0 10 0.100 9.0 0 0.000 0.0 0 0.000 0.0 
1600         
 100 25 0.250 19.0 22 0.220 18.0 3 0.030 3.0 0 0.000 0.0 
3000         
 89 51 0.573 54.0 4 0.045 4.0 47 0.528 49.0 0 0.000 0.0 
5000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 42 0.420 33.0 27 0.270 24.0 15 0.150 13.0 0 0.000 0.0
Trend: 10.254 3.528 9.713  
Probability: 0.000 0.000 0.000  


Trial #:2   Activation: No Activation    Date: 10/04/1984    Harvest Time: 18.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 2 0.020 1.0 2 0.020 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,4-Dimethoxyaniline hydrochloride 
300         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 13 0.130 12.0 9 0.090 8.0 4 0.040 4.0 0 0.000 0.0 
1000         
 100 51 0.510 32.0 24 0.240 20.0 16 0.160 16.0 11 0.110 2.0
Positive Control: Mitomycin-C 
 0.125     
 100 50 0.500 33.0 36 0.360 26.0 14 0.140 14.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 20 0.400 32.0 14 0.280 26.0 6 0.120 10.0 0 0.000 0.0
Trend: 7.379 5.495 5.440  
Probability: 0.000 0.000 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 10/04/1984    Harvest Time: 18 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,4-Dimethoxyaniline hydrochloride 
1000         
 100 53 0.530 13.0 17 0.170 7.0 6 0.060 5.0 30 0.300 3.0 
1600         
 100 12 0.120 5.0 10 0.100 5.0 2 0.020 1.0 0 0.000 0.0 
3000         
 100 105 1.050 78.0 8 0.080 7.0 78 0.780 62.0 19 0.190 10.0 
5000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 42 0.420 33.0 27 0.270 24.0 15 0.150 13.0 0 0.000 0.0
Trend: 12.306 2.006 11.286  
Probability: 0.000 0.022 0.000  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 12/05/1984    Harvest Time: 14 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,4-Dimethoxyaniline hydrochloride 
250         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 100 4 0.040 2.0 2 0.020 2.0 2 0.020 1.0 0 0.000 0.0 
1000         
 100 10 0.100 7.0 8 0.080 6.0 2 0.020 2.0 0 0.000 0.0 
2000         
 100 22 0.220 13.0 16 0.160 10.0 6 0.060 5.0 0 0.000 0.0 
3000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
15         
 100 48 0.480 35.0 19 0.190 16.0 28 0.280 21.0 1 0.010 1.0
Trend: 5.123 4.441 3.045  
Probability: 0.000 0.000 0.001