Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01226-0007-0000-0
Chemical Name:	2,4-Dimethoxyaniline hydrochloride
CASRN:	54150-69-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	832423
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	55	98	99	60	51
			69	101	119	58
			84	104	98	39
			68	97	98	48
Test Chemical	2,4-Dimethoxyaniline hydrochloride	18.75	78	87	102	44	42
			86	95	103	40	42
		37.5	54	103	108	66	53
			75	113	87	39	53
		75	57	85	88	52	58
			69	86	132	64	58
		150	55	44	231	139	122*
			65	39	206	105	122*
		300

Positive Control	Methyl Methane Sulfonate	15	38	30	290	253	216*
Positive Control			50	32	269	179	216*

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	100	94	117	39	40
			103	95	82	27
			88	103	126	48
			81	108	109	45
Test Chemical	2,4-Dimethoxyaniline hydrochloride	25	90	100	86	32	34
			80	93	89	37	34
		75	81	86	57	23	34
			89	93	121	45	34
		125	85	46	153	60	61
			91	45	170	63	61
		175	27	4	197	245	237*
			22	3	153	230	237*
		225

Positive Control	Methyl Methane Sulfonate	15	32	19	99	104	99*
Positive Control			35	26	99	94	99*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	86	49	24	28
			80	85	71	30
			67	99	68	34
			84	130	59	23
Test Chemical	2,4-Dimethoxyaniline hydrochloride	100	82	84	99	40	39
			71	59	79	37	39
		125	78	48	153	65	59*
			82	49	127	52	59*
		150	65	27	184	95	97*
			74	30	220	100	97*
		175	55	13	319	194	198*
			45	11	273	203	198*
		200	26	4	349	456	373*
			50	8	437	290	373*
		225

Positive Control	Methyl Methane Sulfonate	15	54	28	153	94	93*
			45	26	124	92	93*
	Methyl Methane Sulfonate	250	69	67	351	170	165*
			72	85	345	161	165*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination