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Study Information
Study Attribute Value
CEBS Accession Number: 002-01226-0007-0000-0
Chemical Name: 2,4-Dimethoxyaniline hydrochloride
CASRN: 54150-69-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 832423
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 55 98 99 60 51
    69 101 119 58
    84 104 98 39
    68 97 98 48
Test Chemical 2,4-Dimethoxyaniline hydrochloride 
18.75      
 78 87 102 44 42
    86 95 103 40
  
37.5       
 54 103 108 66 53
    75 113 87 39
  
75         
 57 85 88 52 58
    69 86 132 64
  
150         
 55 44 231 139 122*
    65 39 206 105
  
300         
   
Positive Control Methyl Methane Sulfonate 
15         
 38 30 290 253 216*
    50 32 269 179

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 100 94 117 39 40
    103 95 82 27
    88 103 126 48
    81 108 109 45
Test Chemical 2,4-Dimethoxyaniline hydrochloride 
25         
 90 100 86 32 34
    80 93 89 37
  
75         
 81 86 57 23 34
    89 93 121 45
  
125         
 85 46 153 60 61
    91 45 170 63
  
175         
 27 4 197 245 237*
    22 3 153 230
  
225         
   
Positive Control Methyl Methane Sulfonate 
15         
 32 19 99 104 99*
    35 26 99 94

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 69 86 49 24 28
    80 85 71 30
    67 99 68 34
    84 130 59 23
Test Chemical 2,4-Dimethoxyaniline hydrochloride 
100         
 82 84 99 40 39
    71 59 79 37
  
125         
 78 48 153 65 59*
    82 49 127 52
  
150         
 65 27 184 95 97*
    74 30 220 100
  
175         
 55 13 319 194 198*
    45 11 273 203
  
200         
 26 4 349 456 373*
    50 8 437 290
  
225         
   
Positive Control Methyl Methane Sulfonate 
15         
 54 28 153 94 93*
    45 26 124 92
Methyl Methane Sulfonate 
250         
 69 67 351 170 165*
    72 85 345 161

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination