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Study Information
Study Attribute Value
CEBS Accession Number: 002-01228-0001-0000-6
Chemical Name: 2,4-Dinitroaniline
CASRN: 97-02-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 346627_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 08/08/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 474 0.454 9.48 25.5 0.000
Test Chemical 2,4-Dinitroaniline 
 0.2       
 50 1034 479 0.463 9.58 31.6 2.032 
 0.5       
 50 1044 530 0.508 10.60 31.6 11.814 
1.7       
 50 1035 516 0.499 10.32 31.6 9.807 
5         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1049 684 0.652 13.68 25.5 43.616
Mitomycin-C 
 0.01      
 5 104 223 2.144 44.60 25.5 372.274
Trend: 1.795        
Probability: 0.036        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 08/08/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1036 450 0.434 9.00 25.5 0.000
Test Chemical 2,4-Dinitroaniline 
50         
 50 1032 441 0.427 8.82 25.5 -1.620 
166.7       
 50 1037 471 0.454 9.42 31.6 4.566 
500         
 50 1035 577 0.557 11.54 31.6 28.346 
1700         
 0 0 0 0.000 0.00 31.6 N/A 
5000         
 50 1030 477 0.463 9.54 31.6 6.617
Positive Control Cyclophosphamide 
 0.4       
 50 1038 709 0.683 14.18 25.5 57.252
Cyclophosphamide 
2         
 5 103 183 1.777 36.60 25.5 309.036
Trend: 2.241        
Probability: 0.013        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 08/29/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1036 462 0.446 9.24 27.4 0.000
Test Chemical 2,4-Dinitroaniline 
249         
 50 1037 475 0.458 9.50 27.4 2.715 
497.6       
 50 1037 441 0.425 8.82 33.6 -4.638 
753.8       
 0 0 0 0.000 0.00 33.6 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1044 583 0.558 11.66 27.4 25.223
Cyclophosphamide 
2         
 5 104 177 1.702 35.40 27.4 281.643
Trend: -0.763        
Probability: 0.777