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Study Information
Study Attribute Value
CEBS Accession Number: 002-01228-0005-0000-0
Chemical Name: 2,4-Dinitroaniline
CASRN: 97-02-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 044132
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 84 100 61 24 28
    74 98 68 31
    83 113 90 36
    90 89 53 20
Test Chemical 2,4-Dinitroaniline 
30         
 93 112 43 15 17
    91 102 48 18
  
60         
 94 100 94 33 32
    84 89 79 31
  
90         
 95 85 83 29 33
    89 84 97 37
  
120         
 94 84 63 22 25
    104 89 84 27
  
150         
 94 49 54 19 19
    77 52 45 20
Positive Control Methyl Methane Sulfonate 
15         
 60 40 137 77 75*
    58 33 128 73

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 66 80 71 36 31
    82 110 60 24
    89 112 77 29
    82 98 84 34
Test Chemical 2,4-Dinitroaniline 
30         
 70 74 71 34 40
    79 86 110 46
  
60         
 80 87 50 21 21
    83 70 52 21
  
90         
 74 77 65 29 36
    62 69 78 42
  
120         
 72 69 56 26 22
    71 55 37 17
  
150         
 61 59 60 33 25
    89 71 48 18
Positive Control Methyl Methane Sulfonate 
20         
 34 25 160 158 148*
    39 24 159 137
Methyl Methane Sulfonate 
250         
 72 70 374 174 189*
    67 71 411 204

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 55 89 96 59 69
    61 91 149 81
    59 89 128 72
    71 131 139 66
Test Chemical 2,4-Dinitroaniline 
38.5       
 64 91 105 55 54
    68 86 109 53
  
57.8       
 71 96 142 67 69
    66 94 141 71
  
86.7       
 63 81 151 80 71
    82 78 150 61
  
130         
 72 92 164 76 73
    53 78 112 71
  
195         
 55 73 142 86 82
    45 60 105 77
Positive Control 3-Methylcholanthrene 
2.5       
 29 35 380 432 405*
    37 45 420 378

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 82 64 24 28
    102 105 84 27
    86 110 85 33
    110 103 93 28
Test Chemical 2,4-Dinitroaniline 
75         
 110 121 126 38 40
    105 111 135 43
  
100         
 85 104 124 49 48*
    90 117 128 47
  
125         
 95 102 105 37 34
    108 97 99 31
  
150         
 75 73 92 41 40
    101 85 118 39
  
175         
 91 84 119 44 43
    94 87 119 42
Positive Control 3-Methylcholanthrene 
2.5       
 62 40 781 419 330*
    74# 42 537 241

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination