COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-01230-0001-0000-9
Chemical Name: 2,4-Dinitrotoluene
CASRN: 121-14-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 322897_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Negative 
Induced Rat Liver S9  1_S9  Questionable 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 12/27/1984    Trial Call: Negative   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1047 440 0.420 8.80 26.5 0.000
Test Chemical 2,4-Dinitrotoluene
10         
50 1047 472 0.451 9.44 26.5 7.273
30         
50 1050 476 0.453 9.52 30.5 7.873
100         
50 1047 480 0.458 9.60 30.5 9.091
Positive Control Mitomycin-C
 0.0015    
50 1051 738 0.702 14.76 26.5 67.089
Mitomycin-C
 0.01      
10 210 362 1.724 36.20 26.5 310.188
Trend: 1.268        
Probability: 0.102        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 12/27/1984    Trial Call: Questionable   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1047 474 0.453 9.48 25.5 0.000
Test Chemical 2,4-Dinitrotoluene
100         
50 1047 503 0.480 10.06 25.5 6.118
300         
50 1048 518 0.494 10.36 25.5 9.178
1000         
50 1044 564 0.540 11.28 25.5 19.329
Positive Control Cyclophosphamide
 0.5       
50 1046 762 0.728 15.24 25.5 60.913
Cyclophosphamide
2.5       
10 210 300 1.429 30.00 25.5 215.552
Trend: 2.859        
Probability: 0.002        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 02/05/1985    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1054 408 0.387 8.16 26.0 0.000
Test Chemical 2,4-Dinitrotoluene
840         
50 1049 439 0.418 8.78 26.0 8.111
1010         
50 1047 488 0.466 9.76 26.0 20.408
1260         
50 1048 504 0.481 10.08 26.0 24.237
Positive Control Cyclophosphamide
 0.5       
50 1047 760 0.726 15.20 26.0 87.520
Cyclophosphamide
2.5       
10 208 445 2.139 44.50 26.0 452.684
Trend: 3.579        
Probability: 0.000