Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01230-0001-0000-9 |
Chemical Name: | 2,4-Dinitrotoluene |
CASRN: | 121-14-2 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 322897_SCE |
Study Result: | Positive |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Negative |
Induced Rat Liver S9 | 1_S9 | Questionable |
Induced Rat Liver S9 | 2_S9 | Positive |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1047 | 440 | 0.420 | 8.80 | 26.5 | 0.000 |
Test Chemical | 2,4-Dinitrotoluene | 10 | 50 | 1047 | 472 | 0.451 | 9.44 | 26.5 | 7.273 |
30 | 50 | 1050 | 476 | 0.453 | 9.52 | 30.5 | 7.873 | ||
100 | 50 | 1047 | 480 | 0.458 | 9.60 | 30.5 | 9.091 | ||
Positive Control | Mitomycin-C | 0.0015 | 50 | 1051 | 738 | 0.702 | 14.76 | 26.5 | 67.089 |
Mitomycin-C | 0.01 | 10 | 210 | 362 | 1.724 | 36.20 | 26.5 | 310.188 | |
Trend: | 1.268 | ||||||||
Probability: | 0.102 |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1047 | 474 | 0.453 | 9.48 | 25.5 | 0.000 |
Test Chemical | 2,4-Dinitrotoluene | 100 | 50 | 1047 | 503 | 0.480 | 10.06 | 25.5 | 6.118 |
300 | 50 | 1048 | 518 | 0.494 | 10.36 | 25.5 | 9.178 | ||
1000 | 50 | 1044 | 564 | 0.540 | 11.28 | 25.5 | 19.329 | ||
Positive Control | Cyclophosphamide | 0.5 | 50 | 1046 | 762 | 0.728 | 15.24 | 25.5 | 60.913 |
Cyclophosphamide | 2.5 | 10 | 210 | 300 | 1.429 | 30.00 | 25.5 | 215.552 | |
Trend: | 2.859 | ||||||||
Probability: | 0.002 |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1054 | 408 | 0.387 | 8.16 | 26.0 | 0.000 |
Test Chemical | 2,4-Dinitrotoluene | 840 | 50 | 1049 | 439 | 0.418 | 8.78 | 26.0 | 8.111 |
1010 | 50 | 1047 | 488 | 0.466 | 9.76 | 26.0 | 20.408 | ||
1260 | 50 | 1048 | 504 | 0.481 | 10.08 | 26.0 | 24.237 | ||
Positive Control | Cyclophosphamide | 0.5 | 50 | 1047 | 760 | 0.726 | 15.20 | 26.0 | 87.520 |
Cyclophosphamide | 2.5 | 10 | 208 | 445 | 2.139 | 44.50 | 26.0 | 452.684 | |
Trend: | 3.579 | ||||||||
Probability: | 0.000 |