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Study Information
Study Attribute Value
CEBS Accession Number: 002-01230-0011-0000-0
Chemical Name: 2,4-Dinitrotoluene
CASRN: 121-14-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 693724
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 67 84 52 26 21
    72 63 51 24
    115 166 45 13
    88# 87 60 23
Test Chemical 2,4-Dinitrotoluene 
25         
 111 155 61 18 15
    104 94 37 12
    98 103 44 15
  
50         
 55 40 54 33 26
    82# 85 61 25
    65 75 41 21
  
75         
 66 84 50 25 20
    102 126 52 17
    87 100 49 19
  
100         
 88 87 52 20 19
    88 103 40 15
    64 73 41 22
  
150         
 80 78 40 17 20
    94 69 67 24
    82 48 45 18
  
200         
 100 57 62 21 21
    90 72 65 24
    107 63 57 18
  
300         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 56 28 202 120 171*
    48 12 387 270
    87 34 322 123

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 120 92 119 33 34
    121r 67 134 37
    106 113 84 26
    89 95 110 41
Test Chemical 2,4-Dinitrotoluene 
50         
 100 58 119 40 45
    114 62 138 40
    114 62 186 54
  
75         
 122r 64 124 34
    120r 60 183 51
    118 55 162 46
  
100         
 122r 69 125 34
    118 65 130 37
    123r 50 145 39
  
150         
 105 54 131 41 42
    113 57 148 44
    126r 59 137 36
  
200         
 140r 53 218 52 55*
    119 33 229 64
    117 45 163 47
  
300         
 101 20 224 74 101*
    105 9 308 98
    76 4 301 132
Positive Control Methyl Methane Sulfonate 
5         
 73# 36 342 155 280*
    55 28 543 330
    63 24 675 355

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 88 101 185 70 49
    89 103 91 34
    75 96 98 43
Test Chemical 2,4-Dinitrotoluene 
50         
 101 93 303 100 92*
    87 76 210 81
    98 70 276 94
  
75         
 101 62 285 94 93*
    93 79 266 95
    102 95 273 89
  
100         
 75 51 315 141 117*
    116 66 315 90
    69 37 246 119
  
150         
 86 44 191 74 97*
    87 32 247 95
    90 37 326 121
  
200         
 103 34 272 88 99*
    85 34 230 90
    65 21 230 117
  
250         
 70 16 287 136 202*
    99 15 570 192
    76 8 632 277
Positive Control Methyl Methane Sulfonate 
5         
 85 59 695 273 261*
    93 86 641 230
    87 79 727 279

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 112 90 66 20 21
    118 117 73 21
    118 90 69 19
    115 104 80 23
Test Chemical 2,4-Dinitrotoluene 
75         
 121r 69 89 24 20
    94 54 62 22
    93 62 48 17
  
100         
 114 53 90 26 22
    105 62 52 17
    119 64 86 24
  
150         
 107 56 57 18 20
    98 55 72 25
    109 54 61 19
  
200         
 124r 43 97 26
    126r 46 92 24
    112 54 67 20
  
250         
 118 42 97 27 30
    125r 32 160 43
    105 46 102 32
  
300         
 99 30 75 25 46*
    92 13 164 60
    117 14 186 53
Positive Control Methyl Methane Sulfonate 
5         
 94 85 259 92 105*
    118 59 385 108
    84 62 284 113

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 110 112 130 40 36
    114 92 94 27
    112 84 144 43
    100 113 101 34
Test Chemical 2,4-Dinitrotoluene 
12.5       
 107 61 56 17 28
    108 74 126 39
  
25         
 106 67 122 38 39
    102 58 118 39
  
50         
 108 59 114 35 38
    104 68 127 41
  
75         
 118 77 123 35 25
    115 79 61 18
    109 54 78 24
  
100         
 91 32 121 44 48
    82 29 146 60
    113 56 137 40
Positive Control 3-Methylcholanthrene 
2.5       
 103 94 376 122 146*
    98 103 388 132
    111 95 608 183

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 106 111 148 47 51
    79 98 150 63
    75 92 96 43
    121r 146 86 24
Test Chemical 2,4-Dinitrotoluene 
25         
 113 97 103 30 33
    109 88 121 37
    102# 132 93 30
  
50         
 111 132 114 34 35
    93 121 126 45
    117 152 94 27
  
75         
 108 104 136 42 44
    117 99 140 40
    85 102 124 49
  
100         
 102 61 161 53 42
    93 87 125 45
    102 99 88 29
Positive Control 3-Methylcholanthrene 
2.5       
 82 83 672 274 249*
    110 108 834 252
    95 96 634 222

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 7r 8 59 303 69
    74 94 134 60
    96 126 149 52
    54 80 151 94
Test Chemical 2,4-Dinitrotoluene 
25         
 114 72 231 68 84
    115 67 197 57
    94 86 359 127
  
50         
 100 61 197 66 78
    102 74 293 95
    97 70 211 72
  
75         
 104 54 243 78 76
    120r 51 262 73
    92 60 204 74
  
100         
 120r 42 319 88 108*
    92 55 324 118
    90 46 267 99
  
150         
 120 37 395 110 117*
    115 40 436 126
    90 37 308 114
  
200         
 109 24 415 127 161*
    94 23 373 133
    96 15 647 225
Positive Control 3-Methylcholanthrene 
2.5       
 91 70 772 284 543*
    82 64 722 295
    15 11 467 1049

Induced S9 Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 123r 136 254 69 51
    127r 108 183 48
    112 96 158 47
    116 104 188 54
Test Chemical 2,4-Dinitrotoluene 
25         
 113 76 277 81 83*
    100 68 254 85
    154r 102 257 56
  
50         
 115 82 258 75 78
    114 83 281 82
    122r 80 269 74
  
75         
 115 58 334 97
    134r 61 380 95
    122r 69 298 82
  
100         
 140r 83 237 56
    127r 67 328 86
    151r 58 366 81
  
150         
 98 15 396 134 106*
    117 17 429 122
    109 71 202 62
  
200         
 90 7 488 182 171*
    91 6 468 171
    93 13 441 159
Positive Control 3-Methylcholanthrene 
2.5       
 122r 89 704 192 210*
    118 89 746 210
    121r 103 845 232

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination