Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01230-0011-0000-0
Chemical Name:	2,4-Dinitrotoluene
CASRN:	121-14-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	693724
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	67	84	52	26	21
			72	63	51	24
			115	166	45	13
			88#	87	60	23
Test Chemical	2,4-Dinitrotoluene	25	111	155	61	18	15
			104	94	37	12
			98	103	44	15
		50	55	40	54	33	26
			82#	85	61	25
			65	75	41	21
		75	66	84	50	25	20
			102	126	52	17
			87	100	49	19
		100	88	87	52	20	19
			88	103	40	15
			64	73	41	22
		150	80	78	40	17	20
			94	69	67	24
			82	48	45	18
		200	100	57	62	21	21
			90	72	65	24
			107	63	57	18
		300


Positive Control	Methyl Methane Sulfonate	5	56	28	202	120	171*
			48	12	387	270
			87	34	322	123

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	120	92	119	33	34
			121r	67	134	37
			106	113	84	26
			89	95	110	41
Test Chemical	2,4-Dinitrotoluene	50	100	58	119	40	45
			114	62	138	40
			114	62	186	54
		75	122r	64	124	34
			120r	60	183	51
			118	55	162	46
		100	122r	69	125	34
			118	65	130	37
			123r	50	145	39
		150	105	54	131	41	42
			113	57	148	44
			126r	59	137	36
		200	140r	53	218	52	55*
			119	33	229	64
			117	45	163	47
		300	101	20	224	74	101*
			105	9	308	98
			76	4	301	132
Positive Control	Methyl Methane Sulfonate	5	73#	36	342	155	280*
			55	28	543	330
			63	24	675	355

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	88	101	185	70	49
			89	103	91	34
			75	96	98	43
Test Chemical	2,4-Dinitrotoluene	50	101	93	303	100	92*
			87	76	210	81
			98	70	276	94
		75	101	62	285	94	93*
			93	79	266	95
			102	95	273	89
		100	75	51	315	141	117*
			116	66	315	90
			69	37	246	119
		150	86	44	191	74	97*
			87	32	247	95
			90	37	326	121
		200	103	34	272	88	99*
			85	34	230	90
			65	21	230	117
		250	70	16	287	136	202*
			99	15	570	192
			76	8	632	277
Positive Control	Methyl Methane Sulfonate	5	85	59	695	273	261*
			93	86	641	230
			87	79	727	279

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	112	90	66	20	21
			118	117	73	21
			118	90	69	19
			115	104	80	23
Test Chemical	2,4-Dinitrotoluene	75	121r	69	89	24	20
			94	54	62	22
			93	62	48	17
		100	114	53	90	26	22
			105	62	52	17
			119	64	86	24
		150	107	56	57	18	20
			98	55	72	25
			109	54	61	19
		200	124r	43	97	26
			126r	46	92	24
			112	54	67	20
		250	118	42	97	27	30
			125r	32	160	43
			105	46	102	32
		300	99	30	75	25	46*
			92	13	164	60
			117	14	186	53
Positive Control	Methyl Methane Sulfonate	5	94	85	259	92	105*
			118	59	385	108
			84	62	284	113

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	110	112	130	40	36
			114	92	94	27
			112	84	144	43
			100	113	101	34
Test Chemical	2,4-Dinitrotoluene	12.5	107	61	56	17	28
			108	74	126	39	28
		25	106	67	122	38	39
			102	58	118	39	39
		50	108	59	114	35	38
			104	68	127	41	38
		75	118	77	123	35	25
			115	79	61	18
			109	54	78	24
		100	91	32	121	44	48
			82	29	146	60
			113	56	137	40
Positive Control	3-Methylcholanthrene	2.5	103	94	376	122	146*
			98	103	388	132
			111	95	608	183

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	106	111	148	47	51
			79	98	150	63
			75	92	96	43
			121r	146	86	24
Test Chemical	2,4-Dinitrotoluene	25	113	97	103	30	33
			109	88	121	37
			102#	132	93	30
		50	111	132	114	34	35
			93	121	126	45
			117	152	94	27
		75	108	104	136	42	44
			117	99	140	40
			85	102	124	49
		100	102	61	161	53	42
			93	87	125	45
			102	99	88	29
Positive Control	3-Methylcholanthrene	2.5	82	83	672	274	249*
			110	108	834	252
			95	96	634	222

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	7r	8	59	303	69
			74	94	134	60
			96	126	149	52
			54	80	151	94
Test Chemical	2,4-Dinitrotoluene	25	114	72	231	68	84
			115	67	197	57
			94	86	359	127
		50	100	61	197	66	78
			102	74	293	95
			97	70	211	72
		75	104	54	243	78	76
			120r	51	262	73
			92	60	204	74
		100	120r	42	319	88	108*
			92	55	324	118
			90	46	267	99
		150	120	37	395	110	117*
			115	40	436	126
			90	37	308	114
		200	109	24	415	127	161*
			94	23	373	133
			96	15	647	225
Positive Control	3-Methylcholanthrene	2.5	91	70	772	284	543*
			82	64	722	295
			15	11	467	1049

Induced S9 Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	123r	136	254	69	51
			127r	108	183	48
			112	96	158	47
			116	104	188	54
Test Chemical	2,4-Dinitrotoluene	25	113	76	277	81	83*
			100	68	254	85
			154r	102	257	56
		50	115	82	258	75	78
			114	83	281	82
			122r	80	269	74
		75	115	58	334	97
			134r	61	380	95
			122r	69	298	82
		100	140r	83	237	56
			127r	67	328	86
			151r	58	366	81
		150	98	15	396	134	106*
			117	17	429	122
			109	71	202	62
		200	90	7	488	182	171*
			91	6	468	171
			93	13	441	159
Positive Control	3-Methylcholanthrene	2.5	122r	89	704	192	210*
			118	89	746	210
			121r	103	845	232

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination