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Study Information
Study Attribute Value
CEBS Accession Number: 002-01232-0002-0000-2
Chemical Name: 2,4-Toluene diisocyanate
CASRN: 584-84-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 644663_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 

Trial #:1   Activation: No Activation    Date: 07/03/1985    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 1 0.005 1.0 0 0.000 0.0 1 0.005 1.0 0 0.000 0.0
Test Chemical: 2,4-Toluene diisocyanate 
300         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
500         
 200 4 0.020 2.0 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0 
750         
 200 3 0.015 2.0 0 0.000 0.0 3 0.015 2.0 0 0.000 0.0 
1000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 200 37 0.185 15.0 14 0.070 7.0 23 0.115 10.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 8 0.160 14.0 1 0.020 2.0 6 0.120 12.0 1 0.020 1.0
Trend: 1.491 0.721 1.303  
Probability: 0.068 0.235 0.096  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 07/03/1985    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 5 0.025 3.0 2 0.010 1.0 3 0.015 2.0 0 0.000 0.0
Test Chemical: 2,4-Toluene diisocyanate 
300         
 200 1 0.005 1.0 0 0.000 0.0 1 0.005 1.0 0 0.000 0.0 
500         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
750         
 200 1 0.005 1.0 1 0.005 1.0 0 0.000 0.0 0 0.000 0.0 
1000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 50 0.250 21.0 13 0.065 7.0 37 0.185 17.0 0 0.000 0.0
Cyclophosphamide 
10         
 50 19 0.380 36.0 5 0.100 10.0 14 0.280 28.0 0 0.000 0.0
Trend: -2.000 0.004 -2.579  
Probability: 0.977 0.498 0.995  


Trial #:2   Activation: No Activation    Date: 08/07/1985    Harvest Time: 17.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2,4-Toluene diisocyanate 
500         
 200 1 0.005 1.0 0 0.000 0.0 1 0.005 1.0 0 0.000 0.0 
750         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
1000         
 200 3 0.015 2.0 2 0.010 1.0 1 0.005 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.063     
 200 94 0.470 32.0 47 0.235 20.0 37 0.185 16.0 10 0.050 1.0
Mitomycin-C 
 0.125     
 50 47 0.940 62.0 28 0.560 42.0 19 0.380 34.0 0 0.000 0.0
Trend: 1.871 1.812 1.110  
Probability: 0.031 0.035 0.134