Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01232-0004-0000-4
Chemical Name:	2,4-Toluene diisocyanate
CASRN:	584-84-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	445615
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	64	94	59	31	26
			71	99	65	31
			73	102	39	18
			71	104	49	23
Test Chemical	2,4-Toluene diisocyanate	6.25	76	108	75	33	30
			76	103	63	28	30
		12.5	87	81	46	18	21
			73	78	54	25	21
		25	95	94	79	28	26
			77	86	56	24	26
		50	80	59	59	24	27
			100	49	88	29	27
		100	85	42	66	26	26
			80	42	63	26	26
		200
		400

Positive Control	Methyl Methane Sulfonate	15	24	17	123	174	148*
Positive Control			30	19	109	122	148*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	107	84	30	26
			92	94	72	26
			106	104	58	18
			98	95	85	29
Test Chemical	2,4-Toluene diisocyanate	50	85	72	91	36	30
			91	72	68	25	30
		75	80	55	39	16	22
			79	61	64	27	22
		100	112	41	66	20	18
			89	34	43	16	18
		125	79	30	59	25	26
			68	31	53	26	26
		150	63	7	124	65	79*
			56	7	155	92	79*
Positive Control	Methyl Methane Sulfonate	15	62	38	169	91	112*
Positive Control			54	32	214	133	112*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	102	137	64	53
			70	98	118	56
			76	90	100	44
			81	110	121	50
Test Chemical	2,4-Toluene diisocyanate	50	78	91	110	47	44
			73	100	89	40	44
		75	81	47	155	64	64
			82	37	158	65	64
		100	76	30	136	59	67
			79	31	178	75	67
		125	73	27	205	93	82
			65	18	139	71	82
		150	66	20	207	105	121*
			39	10	162	138	121*
Positive Control	3-Methylcholanthrene	2.5	54	31	698	428	437*
Positive Control			52	22	702	446	437*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	68	98	68	33	26
			72	102	65	30
			74	102	36	16
			66	98	45	23
Test Chemical	2,4-Toluene diisocyanate	50	56	47	52	31	38
			58	52	77	44	38
		75	45	25	76	56	61*
			55	38	109	66	61*
		100	35	14	92	87	80*
			47	18	103	73	80*
		125	53	29	86	54	77*
			50	14	149	99	77*
		150	36	5	110	101	131*
			30	4	147	162	131*
Positive Control	3-Methylcholanthrene	2.5	34	26	193	192	210*
Positive Control			36	21	246	228	210*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination