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Study Information
Study Attribute Value
CEBS Accession Number: 002-01244-0001-0000-4
Chemical Name: 2,5-Toluenediamine sulfate
CASRN: 6369-59-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 951026_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 09/12/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1043 410 0.393 8.20 26.5 0.000
Dimethyl Sulfoxide 
0         
 0 0 0 0.000 0.00 0.0 N/A
Test Chemical 2,5-Toluenediamine sulfate 
 0.5       
 50 1050 534 0.509 10.68 26.5 0.000 
1.6       
 50 1049 524 0.500 10.48 26.5 0.000 
5         
 50 1045 553 0.529 11.06 26.5 0.000 
16         
 50 1044 759 0.727 15.18 26.5 0.000 
50         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1043 636 0.610 12.72 26.5 0.000
Mitomycin-C 
 0.005     
 10 208 296 1.423 29.60 26.5 0.000
Trend: 6.587        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 09/25/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 468 0.447 9.36 27.5 0.000
Test Chemical 2,5-Toluenediamine sulfate 
2.5       
 50 1045 495 0.474 9.90 27.5 6.073 
5         
 50 1050 545 0.519 10.90 27.5 16.231 
10         
 50 1048 568 0.542 11.36 27.5 21.368 
20         
 50 1045 658 0.630 13.16 27.5 41.002 
40         
 5 105 68 0.648 13.60 33.5 45.022
Positive Control Mitomycin-C 
 0.0E-4    
 50 1048 626 0.597 12.52 27.5 33.761
Mitomycin-C 
 0.005     
 10 210 341 1.624 34.10 27.5 263.622
Trend: 6.381        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 10/22/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1051 419 0.399 8.38 27.0 0.000
Test Chemical 2,5-Toluenediamine sulfate 
10         
 50 1046 565 0.540 11.30 27.0 35.489 
20         
 50 1044 796 0.762 15.92 27.0 91.250 
30         
 50 1046 1070 1.023 21.40 32.0 156.591 
40         
 50 1049 1206 1.150 24.12 32.0 188.377
Positive Control Mitomycin-C 
 0.001     
 50 1048 737 0.703 14.74 27.0 76.399
Mitomycin-C 
 0.005     
 10 210 334 1.590 33.40 27.0 298.948
Trend: 22.999        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 09/12/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1051 456 0.434 9.12 26.5 0.000
Test Chemical 2,5-Toluenediamine sulfate 
50         
 50 1051 514 0.489 10.28 26.5 12.719 
100         
 50 1047 518 0.495 10.36 26.5 14.030 
150         
 50 1041 513 0.493 10.26 26.5 13.581
Positive Control Cyclophosphamide 
 0.3       
 50 1047 748 0.714 14.96 26.5 64.662
Cyclophosphamide 
 0.6       
 10 209 216 1.033 21.60 26.5 138.202
Trend: 1.947        
Probability: 0.026        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 09/25/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1052 453 0.431 9.06 25.5 0.000
Test Chemical 2,5-Toluenediamine sulfate 
25         
 50 1048 465 0.444 9.30 25.5 3.041 
50         
 50 1048 509 0.486 10.18 25.5 12.791 
100         
 50 1048 501 0.478 10.02 25.5 11.018 
150         
 50 1048 496 0.473 9.92 25.5 9.910
Positive Control Cyclophosphamide 
 0.3       
 50 1050 721 0.687 14.42 25.5 59.464
Cyclophosphamide 
 0.6       
 10 208 187 0.899 18.70 25.5 108.783
Trend: 1.871        
Probability: 0.031