Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01244-0001-0000-4 |
Chemical Name: | 2,5-Toluenediamine sulfate |
CASRN: | 6369-59-1 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 951026_SCE |
Study Result: | Positive |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Positive |
No Activation | 2 | Positive |
No Activation | 3 | Positive |
Induced Rat Liver S9 | 1_S9 | Negative |
Induced Rat Liver S9 | 2_S9 | Negative |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Negative (Not Specified) | 0 | 50 | 1043 | 410 | 0.393 | 8.20 | 26.5 | 0.000 |
Dimethyl Sulfoxide | 0 | 0 | 0 | 0 | 0.000 | 0.00 | 0.0 | N/A | |
Test Chemical | 2,5-Toluenediamine sulfate | 0.5 | 50 | 1050 | 534 | 0.509 | 10.68 | 26.5 | 0.000 |
1.6 | 50 | 1049 | 524 | 0.500 | 10.48 | 26.5 | 0.000 | ||
5 | 50 | 1045 | 553 | 0.529 | 11.06 | 26.5 | 0.000 | ||
16 | 50 | 1044 | 759 | 0.727 | 15.18 | 26.5 | 0.000 | ||
50 | 0 | 0 | 0 | 0.000 | 0.00 | 0.0 | N/A | ||
Positive Control | Mitomycin-C | 0.001 | 50 | 1043 | 636 | 0.610 | 12.72 | 26.5 | 0.000 |
Mitomycin-C | 0.005 | 10 | 208 | 296 | 1.423 | 29.60 | 26.5 | 0.000 | |
Trend: | 6.587 | ||||||||
Probability: | 0.000 |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1048 | 468 | 0.447 | 9.36 | 27.5 | 0.000 |
Test Chemical | 2,5-Toluenediamine sulfate | 2.5 | 50 | 1045 | 495 | 0.474 | 9.90 | 27.5 | 6.073 |
5 | 50 | 1050 | 545 | 0.519 | 10.90 | 27.5 | 16.231 | ||
10 | 50 | 1048 | 568 | 0.542 | 11.36 | 27.5 | 21.368 | ||
20 | 50 | 1045 | 658 | 0.630 | 13.16 | 27.5 | 41.002 | ||
40 | 5 | 105 | 68 | 0.648 | 13.60 | 33.5 | 45.022 | ||
Positive Control | Mitomycin-C | 0.0E-4 | 50 | 1048 | 626 | 0.597 | 12.52 | 27.5 | 33.761 |
Mitomycin-C | 0.005 | 10 | 210 | 341 | 1.624 | 34.10 | 27.5 | 263.622 | |
Trend: | 6.381 | ||||||||
Probability: | 0.000 |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1051 | 419 | 0.399 | 8.38 | 27.0 | 0.000 |
Test Chemical | 2,5-Toluenediamine sulfate | 10 | 50 | 1046 | 565 | 0.540 | 11.30 | 27.0 | 35.489 |
20 | 50 | 1044 | 796 | 0.762 | 15.92 | 27.0 | 91.250 | ||
30 | 50 | 1046 | 1070 | 1.023 | 21.40 | 32.0 | 156.591 | ||
40 | 50 | 1049 | 1206 | 1.150 | 24.12 | 32.0 | 188.377 | ||
Positive Control | Mitomycin-C | 0.001 | 50 | 1048 | 737 | 0.703 | 14.74 | 27.0 | 76.399 |
Mitomycin-C | 0.005 | 10 | 210 | 334 | 1.590 | 33.40 | 27.0 | 298.948 | |
Trend: | 22.999 | ||||||||
Probability: | 0.000 |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1051 | 456 | 0.434 | 9.12 | 26.5 | 0.000 |
Test Chemical | 2,5-Toluenediamine sulfate | 50 | 50 | 1051 | 514 | 0.489 | 10.28 | 26.5 | 12.719 |
100 | 50 | 1047 | 518 | 0.495 | 10.36 | 26.5 | 14.030 | ||
150 | 50 | 1041 | 513 | 0.493 | 10.26 | 26.5 | 13.581 | ||
Positive Control | Cyclophosphamide | 0.3 | 50 | 1047 | 748 | 0.714 | 14.96 | 26.5 | 64.662 |
Cyclophosphamide | 0.6 | 10 | 209 | 216 | 1.033 | 21.60 | 26.5 | 138.202 | |
Trend: | 1.947 | ||||||||
Probability: | 0.026 |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1052 | 453 | 0.431 | 9.06 | 25.5 | 0.000 |
Test Chemical | 2,5-Toluenediamine sulfate | 25 | 50 | 1048 | 465 | 0.444 | 9.30 | 25.5 | 3.041 |
50 | 50 | 1048 | 509 | 0.486 | 10.18 | 25.5 | 12.791 | ||
100 | 50 | 1048 | 501 | 0.478 | 10.02 | 25.5 | 11.018 | ||
150 | 50 | 1048 | 496 | 0.473 | 9.92 | 25.5 | 9.910 | ||
Positive Control | Cyclophosphamide | 0.3 | 50 | 1050 | 721 | 0.687 | 14.42 | 25.5 | 59.464 |
Cyclophosphamide | 0.6 | 10 | 208 | 187 | 0.899 | 18.70 | 25.5 | 108.783 | |
Trend: | 1.871 | ||||||||
Probability: | 0.031 |